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Applied and Environmental Microbiology, October 2002, p. 5012-5016, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5012-5016.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Inhibition of Mollicute Growth: an Approach to Development of Selective Media for Specific Mollicutes

S. A. Keçeli* and R. J. Miles{dagger}

Department of Microbiology, Division of Life Sciences, King's College London, London W8 7AH, United Kingdom

Received 15 April 2002/ Accepted 17 July 2002

The energy-generating pathways of Mycoplasma spp. are diverse. Thus, it was predicted that the ability of inhibitors of these pathways to block growth would vary among species. This prediction was tested with 14 Mycoplasma species and potential inhibitors. The greatest differentiation among test species was obtained using fluoride, iodoacetate (IAA), ß-fluoropyruvate (FP), cibacron blue (CB), L-citrulline, and carbonyl cyanide m-chlorophenylhydrazone. A range of other potential inhibitors, including L-arginine analogues, had little inhibitory effect on growth, and D-arginine was shown to be a growth substrate for arginine-hydrolyzing species. Fluoride selectively inhibited the growth of mycoplasmas that were able only to ferment sugars to lactate and/or to hydrolyze arginine. In contrast, IAA was most effective against organic acid-oxidizing species, and L-citrulline inhibited arginine-hydrolyzing species. Mycoplasma verecundum, a species for which energy sources have not been identified, was relatively resistant to FP. Similarly, Acholeplasma laidlawii was distinguished by its CB resistance.

* Corresponding author. Mailing address: Yuvam Akarca Konutlari, Hekimoglu sok, C1-1 Blok, Daire:14, Alikahya, ·Izmit, Turkey. Phone: 00 90 262 319 10 71. Fax: 00 90 262 233 54 61. E-mail: semakeceli{at}ttnet.net.tr or z.sema{at}mailcity.com.

{dagger} Deceased.

Applied and Environmental Microbiology, October 2002, p. 5012-5016, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5012-5016.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Copyright © 2002 by the American Society for Microbiology. All rights reserved.