This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baudart, J. Articles by Prévost, M. Search for Related Content PubMed PubMed Citation Articles by Baudart, J. Articles by Prévost, M. Agricola Articles by Baudart, J. Articles by Prévost, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2002, p. 5057-5063, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5057-5063.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid and Sensitive Enumeration of Viable Diluted Cells of Members of the Family Enterobacteriaceae in Freshwater and Drinking Water

NSERC Industrial Chair on Drinking Water, Civil, Geological and Mining Engineering, École Polytechnique de Montréal, Montréal, Québec, Canada H3C 3A7,¹ US Filter-John Meunier, St. Laurent, Québec, Canada H4S 2B3²

Received 19 February 2002/ Accepted 2 July 2002

Water quality assessment involves the specific, sensitive, and rapid detection of bacterial indicators and pathogens in water samples, including viable but nonculturable (VBNC) cells. This work evaluates the specificity and sensitivity of a new method which combines a fluorescent in situ hybridization (FISH) approach with a physiological assay (direct viable count [DVC]) for the direct enumeration, at the single-cell level, of highly diluted viable cells of members of the family Enterobacteriaceae in freshwater and drinking water after membrane filtration. The approach (DVC-FISH) uses a new direct detection device, the laser scanning cytometer (Scan RDI). Combining the DVC-FISH method on a membrane with Scan RDI detection makes it possible to detect as few as one targeted cell in approximately 10⁸ nontargeted cells spread over the membrane. The ability of this new approach to detect and enumerate VBNC enterobacterial cells in freshwater and drinking water distribution systems was investigated and is discussed.

This article has been cited by other articles:

• Shams, A. M., Rose, L. J., Hodges, L., Arduino, M. J. (2007). Survival of Burkholderia pseudomallei on Environmental Surfaces. Appl. Environ. Microbiol. 73: 8001-8004 [Abstract] [Full Text]  
• Juhna, T., Birzniece, D., Larsson, S., Zulenkovs, D., Sharipo, A., Azevedo, N. F., Menard-Szczebara, F., Castagnet, S., Feliers, C., Keevil, C. W. (2007). Detection of Escherichia coli in Biofilms from Pipe Samples and Coupons in Drinking Water Distribution Networks. Appl. Environ. Microbiol. 73: 7456-7464 [Abstract] [Full Text]  
• Tobe, K., Eller, G., Medlin, L. K. (2006). Automated detection and enumeration for toxic algae by solid-phase cytometry and the introduction of a new probe for Prymnesium parvum (Haptophyta: Prymnesiophyceae). J PLANKTON RES 28: 643-657 [Abstract] [Full Text]  
• West, N. J., Bacchieri, R., Hansen, G., Tomas, C., Lebaron, P., Moreau, H. (2006). Rapid Quantification of the Toxic Alga Prymnesium parvum in Natural Samples by Use of a Specific Monoclonal Antibody and Solid-Phase Cytometry. Appl. Environ. Microbiol. 72: 860-868 [Abstract] [Full Text]  
• Lisle, J. T., Hamilton, M. A., Willse, A. R., McFeters, G. A. (2004). Comparison of Fluorescence Microscopy and Solid-Phase Cytometry Methods for Counting Bacteria in Water. Appl. Environ. Microbiol. 70: 5343-5348 [Abstract] [Full Text]  
• Aurell, H., Catala, P., Farge, P., Wallet, F., Le Brun, M., Helbig, J. H., Jarraud, S., Lebaron, P. (2004). Rapid Detection and Enumeration of Legionella pneumophila in Hot Water Systems by Solid-Phase Cytometry. Appl. Environ. Microbiol. 70: 1651-1657 [Abstract] [Full Text]