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Applied and Environmental Microbiology, October 2002, p. 5082-5095, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5082-5095.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Complete Sequence and Organization of pBtoxis, the Toxin-Coding Plasmid of Bacillus thuringiensis subsp. israelensis

Colin Berry,1* Susan O'Neil,2 Eitan Ben-Dov,3 Andrew F. Jones,1 Lee Murphy,2 Michael A. Quail,2 Mathew T. G. Holden,2 David Harris,2 Arieh Zaritsky,3 and Julian Parkhill2

Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom,1 The Sanger Institute, Wellcome Trust Genome Campus, Hinxton Cambridge, CB10 1SA, United Kingdom,2 Department of Life Sciences, Ben-Gurion University of the Negev, Be’er-Sheva 84105, Israel3

Received 29 April 2002/ Accepted 18 June 2002

The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.


* Corresponding author. Mailing address: Cardiff School of Biosciences, Cardiff University, P.O. Box 911, Museum Ave., Cardiff, CF10 3US, Wales, United Kingdom. Phone: 44-29-20874508. Fax: 44-29-20874486. E-mail: Berry{at}cf.ac.uk.


Applied and Environmental Microbiology, October 2002, p. 5082-5095, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5082-5095.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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Copyright © 2002 by the American Society for Microbiology. All rights reserved.