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Applied and Environmental Microbiology, October 2002, p. 5151-5154, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5151-5154.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Double-Staining Method for Differentiation of Morphological Changes and Membrane Integrity of Campylobacter coli Cells

Jose L. Alonso,1* Salvatore Mascellaro,2 Yolanda Moreno,2 María A. Ferrús,2 and Javier Hernández2

Instituto de Hidrología y Medio Natural,1 Departamento de Biotecnología, Universidad Politécnica, 46022 Valencia, Spain2

Received 26 December 2001/ Accepted 4 July 2002

We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells.

* Corresponding author. Mailing address: Instituto de Hidrología y Medio Natural, Universidad Politécnica, Camino de Vera 14, 46022 Valencia, Spain. Phone: 96-3877090. Fax: 96-3877090. E-mail: jalonso{at}ihdr.upv.es.

Applied and Environmental Microbiology, October 2002, p. 5151-5154, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5151-5154.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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