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Applied and Environmental Microbiology, October 2002, p. 5164-5166, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5164-5166.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Helicobacter Colonization of the Murine Lower Bowel by Genus-Specific PCR-Denaturing Gradient Gel Electrophoresis

Martin Grehan,* Gauri Tamotia, Bronwyn Robertson, and Hazel Mitchell

School of Microbiology and Immunology, The University of New South Wales, Sydney, New South Wales, Australia 2052

Received 7 December 2001/ Accepted 25 July 2002

Helicobacter genus-specific PCR and denaturing gradient gel electrophoresis can detect and speciate the helicobacters that colonize the lower bowel of laboratory mice. The method's sensitivity is comparable to that of species-specific PCR and may detect unnamed Helicobacter species. This approach should prove useful for commercial and research murine facilities.

* Corresponding author. Mailing address: School of Microbiology and Immunology, The University of New South Wales, Sydney, NSW, Australia 2052. Phone: 61 (2) 9385 3514. Fax: 61 (2) 9385 1591. E-mail: m.grehan{at}student.unsw.edu.au.

Applied and Environmental Microbiology, October 2002, p. 5164-5166, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5164-5166.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Nilsson, H.-O., Ouis, I.-S., Stenram, U., Ljungh, A., Moran, A. P., Wadstrom, T., Al-Soud, W. A. (2004). High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR-Denaturing Gradient Gel Electrophoresis and Pyrosequencing. J. Clin. Microbiol. 42: 3781-3788 [Abstract] [Full Text]  
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