This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by del Cerro, A.
Right arrow Articles by Guijarro, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by del Cerro, A.
Right arrow Articles by Guijarro, J. A.
Agricola
Right arrow Articles by del Cerro, A.
Right arrow Articles by Guijarro, J. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2002, p. 5177-5180, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5177-5180.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR

A. del Cerro,1 I. Marquez,2 and J. A. Guijarro1*

Área de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo,1 SERIDA, Laboratorio de Sanidad Animal de Jove, 33299 Gijón, Asturias, Spain2

Received 18 March 2002/ Accepted 4 July 2002

A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.

* Corresponding author. Mailing address: Área de Microbiología, Departamento de Biología Funcional. Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain. Phone: 34985104218. Fax: 34985103148. E-mail: jaga{at}sauron.quimica.uniovi.es.

Applied and Environmental Microbiology, October 2002, p. 5177-5180, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5177-5180.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sanjuan, E., Amaro, C. (2007). Multiplex PCR Assay for Detection of Vibrio vulnificus Biotype 2 and Simultaneous Discrimination of Serovar E Strains. Appl. Environ. Microbiol. 73: 2029-2032 [Abstract] [Full Text]  
  • Balcazar, J. L., Vendrell, D., de Blas, I., Ruiz-Zarzuela, I., Girones, O., Muzquiz, J. L. (2007). Quantitative detection of Aeromonas salmonicida in fish tissue by real-time PCR using self-quenched, fluorogenic primers. J Med Microbiol 56: 323-328 [Abstract] [Full Text]  
  • Warsen, A. E., Krug, M. J., LaFrentz, S., Stanek, D. R., Loge, F. J., Call, D. R. (2004). Simultaneous Discrimination between 15 Fish Pathogens by Using 16S Ribosomal DNA PCR and DNA Microarrays. Appl. Environ. Microbiol. 70: 4216-4221 [Abstract] [Full Text]  
  • Mata, A. I., Gibello, A., Casamayor, A., Blanco, M. M., Dominguez, L., Fernandez-Garayzabal, J. F. (2004). Multiplex PCR Assay for Detection of Bacterial Pathogens Associated with Warm-Water Streptococcosis in Fish. Appl. Environ. Microbiol. 70: 3183-3187 [Abstract] [Full Text]  
  • Gonzalez, S. F., Krug, M. J., Nielsen, M. E., Santos, Y., Call, D. R. (2004). Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray. J. Clin. Microbiol. 42: 1414-1419 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»