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Applied and Environmental Microbiology, November 2002, p. 5241-5248, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5241-5248.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Utilization of Ganglioside-Degrading Paenibacillus sp. Strain TS12 for Production of Glucosylceramide

Tomomi Sumida, Noriyuki Sueyoshi, and Makoto Ito*

Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan

Received 1 April 2002/ Accepted 9 August 2002

Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the production of glucosylceramide with this bacterium. GM1a ganglioside was found to be sequentially degraded by Paenibacillus sp. strain TS12, which was isolated from soil, as follows: GM1a -> asialo GM1 -> asialo GM2 -> lactosylceramide -> glucosylceramide. TS12 was found to produce a series of ganglioside-degrading enzymes, such as sialidases, ß-galactosidases, and ß-hexosaminidases. TS12 also produced ß-glucosidases, but glucosylceramide was somewhat resistant to the bacterial enzyme under the conditions used. Taking advantage of the specificity, we developed a new method for the production of glucosylceramide using TS12 as a biocatalyst. The method involves the conversion of crude bovine brain gangliosides to glucosylceramide by coculture with TS12 and purification of the product by chromatography with Wakogel C-300 HG.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Phone: 81-92-642-2898. Fax: 81-92-642-2898 or 81-92-642-2907. E-mail: makotoi{at}agr.kyushu-u.ac.jp.

Applied and Environmental Microbiology, November 2002, p. 5241-5248, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5241-5248.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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