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Applied and Environmental Microbiology, November 2002, p. 5367-5373, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5367-5373.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny

Mike Manefield, Andrew S. Whiteley, Robert I. Griffiths, and Mark J. Bailey*

CEH Oxford, Oxford OX1 3SR, United Kingdom

Received 24 June 2002/ Accepted 20 August 2002

Identifying microorganisms responsible for recognized environmental processes remains a great challenge in contemporary microbial ecology. Only in the last few years have methodological innovations provided access to the relationship between the function of a microbial community and the phylogeny of the organisms accountable for it. In this study stable-isotope-labeled [13C]phenol was fed into a phenol-degrading community from an aerobic industrial bioreactor, and the 13C-labeled RNA produced was used to identify the bacteria responsible for the process. Stable-isotope-labeled RNA was analyzed by equilibrium density centrifugation in concert with reverse transcription-PCR and denaturing gradient gel electrophoresis. In contradiction with findings from conventional methodologies, this unique approach revealed that phenol degradation in the microbial community under investigation is dominated by a member of the Thauera genus. Our results suggest that this organism is important for the function of this bioreactor.

* Corresponding author. Mailing address: Molecular Microbial Ecology Group, CEH Oxford, Mansfield Rd., Oxford OX1 3SR, United Kingdom. Phone: 44 1865281630. Fax: 44 1865281696. E-mail: mbailey{at}ceh.ac.uk.

Applied and Environmental Microbiology, November 2002, p. 5367-5373, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5367-5373.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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