This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heidelberg, J. F.
Right arrow Articles by Colwell, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heidelberg, J. F.
Right arrow Articles by Colwell, R. R.
Agricola
Right arrow Articles by Heidelberg, J. F.
Right arrow Articles by Colwell, R. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2002, p. 5488-5497, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5488-5497.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Seasonality of Chesapeake Bay Bacterioplankton Species

J. F. Heidelberg,1,{dagger} K. B. Heidelberg,2 and R. R. Colwell1,3*

Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202,1 Department of Cell Biology and Molecular Genetics,2 Department of Biology, University of Maryland, College Park, Maryland 207423

Received 8 March 2002/ Accepted 5 August 2002

Bacteria, {gamma}-subclass of Proteobacteria, Vibrio-Photobacterium, Vibrio vulnificus, Vibrio cholerae-Vibrio mimicus, and Vibrio cincinnatiensis in water samples collected from the Choptank River in Chesapeake Bay from 15 April to 16 December 1996 were enumerated using a fluorescent oligonucleotide direct-counting (FODC) procedure. FODC results obtained using a Bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct count (AODC) procedure. The abundance of individual taxa (per liter) ranged from 0.25 x 1010 to 2.6 x 1010 Bacteria, 0.32 x 108 to 3.1 x 108 {gamma}-Proteobacteria, 0.2 x 108 to 2.1 x 108 Vibrio-Photobacterium, 0.5 x 107 to 10 x 107 V. vulnificus, 0.2 x 106 to 6 x 106 V. cholerae-V. mimicus, and 0.5 x 105 to 8 x 105 V. cincinnatiensis. The occurrence of all taxa monitored in this study was higher in summer; however, these taxa made up a larger proportion of the Bacteria when the water temperature was low. Large fluctuations in species abundance as well as in percent composition of Vibrio-Photobacterium occurred from week to week, indicating that localized blooms of these taxa occur. The cross-Choptank River transect sample profile of V. vulnificus and V. cholerae-V. mimicus varied significantly in abundance, and trans-Choptank River transect samples revealed a patchy distribution.

* Corresponding author. Mailing address: Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt Street, Baltimore, MD 21202. Phone: (703) 292-8000. Fax: (703) 292-9232. E-mail: colwell{at}umbi.umd.edu.

{dagger} Present address: The Institute for Genomic Research (TIGR), Rockville, MD 20850.

Applied and Environmental Microbiology, November 2002, p. 5488-5497, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5488-5497.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Constantin de Magny, G., Murtugudde, R., Sapiano, M. R. P., Nizam, A., Brown, C. W., Busalacchi, A. J., Yunus, M., Nair, G. B., Gil, A. I., Lanata, C. F., Calkins, J., Manna, B., Rajendran, K., Bhattacharya, M. K., Huq, A., Sack, R. B., Colwell, R. R. (2008). From the Cover: Environmental signatures associated with cholera epidemics. Proc. Natl. Acad. Sci. USA 105: 17676-17681 [Abstract] [Full Text]  
  • Rawlings, T. K., Ruiz, G. M., Colwell, R. R. (2007). Association of Vibrio cholerae O1 El Tor and O139 Bengal with the Copepods Acartia tonsa and Eurytemora affinis. Appl. Environ. Microbiol. 73: 7926-7933 [Abstract] [Full Text]  
  • Bench, S. R., Hanson, T. E., Williamson, K. E., Ghosh, D., Radosovich, M., Wang, K., Wommack, K. E. (2007). Metagenomic Characterization of Chesapeake Bay Virioplankton. Appl. Environ. Microbiol. 73: 7629-7641 [Abstract] [Full Text]  
  • Kan, J., Suzuki, M. T., Wang, K., Evans, S. E., Chen, F. (2007). High Temporal but Low Spatial Heterogeneity of Bacterioplankton in the Chesapeake Bay. Appl. Environ. Microbiol. 73: 6776-6789 [Abstract] [Full Text]  
  • Sinigalliano, C. D., Gidley, M. L., Shibata, T., Whitman, D., Dixon, T. H., Laws, E., Hou, A., Bachoon, D., Brand, L., Amaral-Zettler, L., Gast, R. J., Steward, G. F., Nigro, O. D., Fujioka, R., Betancourt, W. Q., Vithanage, G., Mathews, J., Fleming, L. E., Solo-Gabriele, H. M. (2007). Impacts of Hurricanes Katrina and Rita on the microbial landscape of the New Orleans area. Proc. Natl. Acad. Sci. USA 104: 9029-9034 [Abstract] [Full Text]  
  • Eiler, A., Johansson, M., Bertilsson, S. (2006). Environmental Influences on Vibrio Populations in Northern Temperate and Boreal Coastal Waters (Baltic and Skagerrak Seas). Appl. Environ. Microbiol. 72: 6004-6011 [Abstract] [Full Text]  
  • Pernthaler, J., Amann, R. (2005). Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations. Microbiol. Mol. Biol. Rev. 69: 440-461 [Abstract] [Full Text]  
  • Binsztein, N., Costagliola, M. C., Pichel, M., Jurquiza, V., Ramirez, F. C., Akselman, R., Vacchino, M., Huq, A., Colwell, R. (2004). Viable but Nonculturable Vibrio cholerae O1 in the Aquatic Environment of Argentina. Appl. Environ. Microbiol. 70: 7481-7486 [Abstract] [Full Text]  
  • Randa, M. A., Polz, M. F., Lim, E. (2004). Effects of Temperature and Salinity on Vibrio vulnificus Population Dynamics as Assessed by Quantitative PCR. Appl. Environ. Microbiol. 70: 5469-5476 [Abstract] [Full Text]  
  • Thompson, F. L., Iida, T., Swings, J. (2004). Biodiversity of Vibrios. Microbiol. Mol. Biol. Rev. 68: 403-431 [Abstract] [Full Text]  
  • Thompson, J. R., Randa, M. A., Marcelino, L. A., Tomita-Mitchell, A., Lim, E., Polz, M. F. (2004). Diversity and Dynamics of a North Atlantic Coastal Vibrio Community. Appl. Environ. Microbiol. 70: 4103-4110 [Abstract] [Full Text]  
  • Thompson, C. C., Thompson, F. L., Vandemeulebroecke, K., Hoste, B., Dawyndt, P., Swings, J. (2004). Use of recA as an alternative phylogenetic marker in the family Vibrionaceae. Int. J. Syst. Evol. Microbiol. 54: 919-924 [Abstract] [Full Text]  
  • Short, S. M., Jenkins, B. D., Zehr, J. P. (2004). Spatial and Temporal Distribution of Two Diazotrophic Bacteria in the Chesapeake Bay. Appl. Environ. Microbiol. 70: 2186-2192 [Abstract] [Full Text]  
  • Jenkins, B. D., Steward, G. F., Short, S. M., Ward, B. B., Zehr, J. P. (2004). Fingerprinting Diazotroph Communities in the Chesapeake Bay by Using a DNA Macroarray. Appl. Environ. Microbiol. 70: 1767-1776 [Abstract] [Full Text]  
  • Thompson, F. L., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Gullian, M., Swings, J. (2003). Vibrio fortis sp. nov. and Vibrio hepatarius sp. nov., isolated from aquatic animals and the marine environment. Int. J. Syst. Evol. Microbiol. 53: 1495-1501 [Abstract] [Full Text]  
  • Lipp, E. K., Rivera, I. N. G., Gil, A. I., Espeland, E. M., Choopun, N., Louis, V. R., Russek-Cohen, E., Huq, A., Colwell, R. R. (2003). Direct Detection of Vibrio cholerae and ctxA in Peruvian Coastal Water and Plankton by PCR. Appl. Environ. Microbiol. 69: 3676-3680 [Abstract] [Full Text]  
  • Beardsley, C., Pernthaler, J., Wosniok, W., Amann, R. (2003). Are Readily Culturable Bacteria in Coastal North Sea Waters Suppressed by Selective Grazing Mortality?. Appl. Environ. Microbiol. 69: 2624-2630 [Abstract] [Full Text]  
  • Louis, V. R., Russek-Cohen, E., Choopun, N., Rivera, I. N. G., Gangle, B., Jiang, S. C., Rubin, A., Patz, J. A., Huq, A., Colwell, R. R. (2003). Predictability of Vibrio cholerae in Chesapeake Bay. Appl. Environ. Microbiol. 69: 2773-2785 [Abstract] [Full Text]  
  • Thompson, F. L., Thompson, C. C., Vicente, A. C. P., Theophilo, G. N. D., Hofer, E., Swings, J. (2003). Genomic Diversity of Clinical and Environmental Vibrio cholerae Strains Isolated in Brazil between 1991 and 2001 as Revealed by Fluorescent Amplified Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 41: 1946-1950 [Abstract] [Full Text]  
  • Colwell, R. R., Huq, A., Islam, M. S., Aziz, K. M. A., Yunus, M., Khan, N. H., Mahmud, A., Sack, R. B., Nair, G. B., Chakraborty, J., Sack, D. A., Russek-Cohen, E. (2003). Reduction of cholera in Bangladeshi villages by simple filtration. Proc. Natl. Acad. Sci. USA 100: 1051-1055 [Abstract] [Full Text]  
  • Heidelberg, J. F., Heidelberg, K. B., Colwell, R. R. (2002). Bacteria of the {gamma}-Subclass Proteobacteria Associated with Zooplankton in Chesapeake Bay. Appl. Environ. Microbiol. 68: 5498-5507 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»