This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heidelberg, J. F.
Right arrow Articles by Colwell, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heidelberg, J. F.
Right arrow Articles by Colwell, R. R.
Agricola
Right arrow Articles by Heidelberg, J. F.
Right arrow Articles by Colwell, R. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2002, p. 5498-5507, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5498-5507.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bacteria of the {gamma}-Subclass Proteobacteria Associated with Zooplankton in Chesapeake Bay

J. F. Heidelberg,1,{dagger} K. B. Heidelberg,2 and R. R. Colwell1,3*

Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202,1 Department of Cell Biology and Molecular Genetics,3 Department of Biology, University of Maryland, College Park, Maryland 207422

Received 15 March 2002/ Accepted 5 August 2002

The seasonal abundance of {gamma}-subclass Proteobacteria, Vibrio-Photobacterium, Vibrio cholerae-Vibrio mimicus, Vibrio cincinnatiensis, and Vibrio vulnificus in the Choptank River of Chesapeake Bay associated with zooplankton was monitored from April to December 1996. Large (>202-µm) and small (64- to 202-µm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. Large populations of bacteria were found to be associated with both the large and small size classes of zooplankton. Also, the species of bacteria associated with the zooplankton showed seasonal abundance, with the largest numbers occurring in the early spring and again in the summer, when zooplankton total numbers were correspondingly large. Approximately 0.01 to 40.0% of the total water column bacteria were associated with zooplankton, with the percentage of the total water column bacteria population associated with zooplankton varying by season. A taxonomically diverse group of bacteria was associated with zooplankton, and a larger proportion was found in and on zooplankton during the cooler months of the year, with selected taxa comprising a larger percent of the Bacteria in the summer. V. cholerae-V. mimicus and V. vulnificus comprised the bulk of the large and small zooplankton-associated Vibrio-Photobacterium species. In contrast, V. cincinnatiensis accounted for less than 0.1 to 3%. It is concluded that water column and zooplankton bacterial populations vary independently with respect to species composition since no correlation was observed between taxa occurring with highest frequency in the water column and those in association with zooplankton.


* Corresponding author. Mailing address: Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt St., Baltimore, MD 21202. Phone: (703) 292-8000. Fax: (703) 292-9232. E-mail: colwell{at}umbi.umd.edu.

{dagger} Present address: The Institute for Genomic Research (TIGR), Rockville, MD 20850.


Applied and Environmental Microbiology, November 2002, p. 5498-5507, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5498-5507.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Stillman, J. H., Colbourne, J. K., Lee, C. E., Patel, N. H., Phillips, M. R., Towle, D. W., Eads, B. D., Gelembuik, G. W., Henry, R. P., Johnson, E. A., Pfrender, M. E., Terwilliger, N. B. (2008). Recent advances in crustacean genomics. Integr. Comp. Biol. 48: 852-868 [Abstract] [Full Text]  
  • Constantin de Magny, G., Murtugudde, R., Sapiano, M. R. P., Nizam, A., Brown, C. W., Busalacchi, A. J., Yunus, M., Nair, G. B., Gil, A. I., Lanata, C. F., Calkins, J., Manna, B., Rajendran, K., Bhattacharya, M. K., Huq, A., Sack, R. B., Colwell, R. R. (2008). From the Cover: Environmental signatures associated with cholera epidemics. Proc. Natl. Acad. Sci. USA 105: 17676-17681 [Abstract] [Full Text]  
  • Hopkinson, B. M., Roe, K. L., Barbeau, K. A. (2008). Heme Uptake by Microscilla marina and Evidence for Heme Uptake Systems in the Genomes of Diverse Marine Bacteria. Appl. Environ. Microbiol. 74: 6263-6270 [Abstract] [Full Text]  
  • Peter, H., Sommaruga, R. (2008). An evaluation of methods to study the gut bacterial community composition of freshwater zooplankton. J PLANKTON RES 30: 997-1006 [Abstract] [Full Text]  
  • Hunt, D. E., David, L. A., Gevers, D., Preheim, S. P., Alm, E. J., Polz, M. F. (2008). Resource Partitioning and Sympatric Differentiation Among Closely Related Bacterioplankton. Science 320: 1081-1085 [Abstract] [Full Text]  
  • Kirschner, A. K. T., Schlesinger, J., Farnleitner, A. H., Hornek, R., Suss, B., Golda, B., Herzig, A., Reitner, B. (2008). Rapid Growth of Planktonic Vibrio cholerae Non-O1/Non-O139 Strains in a Large Alkaline Lake in Austria: Dependence on Temperature and Dissolved Organic Carbon Quality. Appl. Environ. Microbiol. 74: 2004-2015 [Abstract] [Full Text]  
  • Hunt, D. E., Gevers, D., Vahora, N. M., Polz, M. F. (2008). Conservation of the Chitin Utilization Pathway in the Vibrionaceae. Appl. Environ. Microbiol. 74: 44-51 [Abstract] [Full Text]  
  • Rawlings, T. K., Ruiz, G. M., Colwell, R. R. (2007). Association of Vibrio cholerae O1 El Tor and O139 Bengal with the Copepods Acartia tonsa and Eurytemora affinis. Appl. Environ. Microbiol. 73: 7926-7933 [Abstract] [Full Text]  
  • Cohen, A. L. V., Oliver, J. D., DePaola, A., Feil, E. J., Fidelma Boyd, E. (2007). Emergence of a Virulent Clade of Vibrio vulnificus and Correlation with the Presence of a 33-Kilobase Genomic Island. Appl. Environ. Microbiol. 73: 5553-5565 [Abstract] [Full Text]  
  • Eiler, A., Johansson, M., Bertilsson, S. (2006). Environmental Influences on Vibrio Populations in Northern Temperate and Boreal Coastal Waters (Baltic and Skagerrak Seas). Appl. Environ. Microbiol. 72: 6004-6011 [Abstract] [Full Text]  
  • Thompson, F. L., Iida, T., Swings, J. (2004). Biodiversity of Vibrios. Microbiol. Mol. Biol. Rev. 68: 403-431 [Abstract] [Full Text]  
  • Thompson, J. R., Randa, M. A., Marcelino, L. A., Tomita-Mitchell, A., Lim, E., Polz, M. F. (2004). Diversity and Dynamics of a North Atlantic Coastal Vibrio Community. Appl. Environ. Microbiol. 70: 4103-4110 [Abstract] [Full Text]  
  • Thompson, C. C., Thompson, F. L., Vandemeulebroecke, K., Hoste, B., Dawyndt, P., Swings, J. (2004). Use of recA as an alternative phylogenetic marker in the family Vibrionaceae. Int. J. Syst. Evol. Microbiol. 54: 919-924 [Abstract] [Full Text]  
  • Mourino-Perez, R. R., Worden, A. Z., Azam, F. (2003). Growth of Vibrio cholerae O1 in Red Tide Waters off California. Appl. Environ. Microbiol. 69: 6923-6931 [Abstract] [Full Text]  
  • Thompson, F. L., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Gullian, M., Swings, J. (2003). Vibrio fortis sp. nov. and Vibrio hepatarius sp. nov., isolated from aquatic animals and the marine environment. Int. J. Syst. Evol. Microbiol. 53: 1495-1501 [Abstract] [Full Text]  
  • Louis, V. R., Russek-Cohen, E., Choopun, N., Rivera, I. N. G., Gangle, B., Jiang, S. C., Rubin, A., Patz, J. A., Huq, A., Colwell, R. R. (2003). Predictability of Vibrio cholerae in Chesapeake Bay. Appl. Environ. Microbiol. 69: 2773-2785 [Abstract] [Full Text]  
  • Colwell, R. R., Huq, A., Islam, M. S., Aziz, K. M. A., Yunus, M., Khan, N. H., Mahmud, A., Sack, R. B., Nair, G. B., Chakraborty, J., Sack, D. A., Russek-Cohen, E. (2003). Reduction of cholera in Bangladeshi villages by simple filtration. Proc. Natl. Acad. Sci. USA 100: 1051-1055 [Abstract] [Full Text]  
  • Heidelberg, J. F., Heidelberg, K. B., Colwell, R. R. (2002). Seasonality of Chesapeake Bay Bacterioplankton Species. Appl. Environ. Microbiol. 68: 5488-5497 [Abstract] [Full Text]