This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Refsum, T.
Right arrow Articles by Holstad, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Refsum, T.
Right arrow Articles by Holstad, G.
Agricola
Right arrow Articles by Refsum, T.
Right arrow Articles by Holstad, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2002, p. 5600-5606, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5600-5606.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Salmonella enterica Serovar Typhimurium Isolates Determined by Pulsed-Field Gel Electrophoresis: Comparison of Isolates from Avian Wildlife, Domestic Animals, and the Environment in Norway

Thorbjørn Refsum,1* Even Heir,2 Georg Kapperud,2,3 Traute Vardund,2 and Gudmund Holstad1

Section of Bacteriology, National Veterinary Institute,1 Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, N-0033 Oslo,3 Division of Infectious Disease Control, Norwegian Institute of Public Health, N-0403 Oslo, Norway2

Received 25 February 2002/ Accepted 14 August 2002

The molecular epidemiology of 142 isolates of Salmonella enterica serovar Typhimurium from avian wildlife, domestic animals, and the environment in Norway was investigated using pulsed-field gel electrophoresis (PFGE) and computerized numerical analysis of the data. The bacterial isolates comprised 79 isolates from wild-living birds, including 46 small passerines and 26 gulls, and 63 isolates of nonavian origin, including 50 domestic animals and 13 environmental samples. Thirteen main clusters were discernible at the 90% similarity level. Most of the isolates (83%) were grouped into three main clusters. These were further divided into 20 subclusters at the 95% similarity level. Isolates from passerines, gulls, and pigeons dominated within five subclusters, whereas isolates from domestic animals and the environment belonged to many different subclusters with no predominance. The results support earlier results that passerines constitute an important source of infection to humans in Norway, whereas it is suggested that gulls and pigeons, based on PFGE analysis, represent only a minor source of human serovar Typhimurium infections. Passerines, gulls, and pigeons may also constitute a source of infection of domestic animals and feed plants or vice versa. Three isolates from cattle and a grain source, of which two were multiresistant, were confirmed as serovar Typhimurium phage type DT 104. These represent the first reported phage type DT 104 isolates from other sources than humans in Norway.

* Corresponding author: Mailing address: Section of Bacteriology, National Veterinary Institute, P.O. Box 8156 Dep. N-0033 Oslo, Norway. Phone: 47 23 21 63 22. Fax: 47 23 21 63 01. E-mail: thorbjorn.refsum{at}vetinst.no.

Applied and Environmental Microbiology, November 2002, p. 5600-5606, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5600-5606.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hall, A. J., Saito, E. K. (2008). AVIAN WILDLIFE MORTALITY EVENTS DUE TO SALMONELLOSIS IN THE UNITED STATES, 1985-2004. J Wildl Dis 44: 585-593 [Abstract] [Full Text]  
  • Gaertner, J. P., Hahn, D., Rose, F. L., Forstner, M. R. J. (2008). Detection of Salmonellae in Different Turtle Species within a Headwater Spring Ecosystem. J Wildl Dis 44: 519-526 [Abstract] [Full Text]  
  • Doran, G., Morris, D., O'Hare, C., DeLappe, N., Bradshaw, B., Corbett-Feeney, G., Cormican, M. (2005). Cost-Effective Application of Pulsed-Field Gel Electrophoresis to Typing of Salmonella enterica Serovar Typhimurium. Appl. Environ. Microbiol. 71: 8236-8240 [Abstract] [Full Text]  
  • Refsum, T., Handeland, K., Baggesen, D. L., Holstad, G., Kapperud, G. (2002). Salmonellae in Avian Wildlife in Norway from 1969 to 2000. Appl. Environ. Microbiol. 68: 5595-5599 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»