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Applied and Environmental Microbiology, November 2002, p. 5620-5624, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5620-5624.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of an Anaplerotic Enzyme, Pyruvate Carboxylase, Improves Recombinant Protein Production in Escherichia coli

J. C. March, M. A. Eiteman,* and E. Altman

Center for Molecular BioEngineering, Department of Biological and Agricultural Engineering, University of Georgia, Athens, Georgia 30602

Received 24 June 2002/ Accepted 26 August 2002

Anaplerotic enzyme reactions are those which replenish tricarboxylic acid intermediates that are withdrawn for the synthesis of biomass. In this study, we examined recombinant protein production in Escherichia coli containing activity in an additional anaplerotic enzyme, pyruvate carboxylase. In batch fermentations, the presence of pyruvate carboxylase resulted in 68% greater production of the model protein, ß-galactosidase, 41% greater cell yield, and 57% lower acetate concentration. We discuss why these results indicate that acetate concentration does not limit cell growth and protein synthesis, as predicted by other researchers, and suggest instead that the rate of acetate formation represents an inefficient consumption of glucose carbon, which is reduced by the presence of pyruvate carboxylase.

* Corresponding author. Mailing address: Center for Molecular BioEngineering, Department of Biological and Agricultural Engineering, Driftmier Engineering Center, University of Georgia, Athens, GA 30602. Phone: (706) 542-0833. Fax: (706) 542-8806. E-mail: eiteman{at}engr.uga.edu.

Applied and Environmental Microbiology, November 2002, p. 5620-5624, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5620-5624.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lai, H., Kraszewski, J. L., Purwantini, E., Mukhopadhyay, B. (2006). Identification of Pyruvate Carboxylase Genes in Pseudomonas aeruginosa PAO1 and Development of a P. aeruginosa-Based Overexpression System for {alpha}4- and {alpha}4{beta}4-Type Pyruvate Carboxylases. Appl. Environ. Microbiol. 72: 7785-7792 [Abstract] [Full Text]  


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