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Applied and Environmental Microbiology, November 2002, p. 5704-5710, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5704-5710.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Serracin P, a Phage-Tail-Like Bacteriocin, and Its Activity against Erwinia amylovora, the Fire Blight Pathogen

Abdelhamid Jabrane,1* Ahmed Sabri,1 Philippe Compère,2 Philippe Jacques,1 Isabel Vandenberghe,3 Jozef Van Beeumen,3 and Philippe Thonart1

Centre Wallon de Biologie Industrielle, Service de Technologie Microbienne, Université de Liège, Sart-Tilman, BE-4000 Liège,1 Biologie Générale et Morphologie Ultrastructurale, Université de Liège, BE-4020 Liège,2 Laboratorium voor Eiwitbiochemie en Eiwitengineering, BE-9000 Ghent, Belgium3

Received 7 February 2002/ Accepted 16 July 2002

Serratia plymithicum J7 culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora, the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens, and Pseudomonas fluorescens. This activity increased significantly upon induction with mitomycin C. A phage-tail-like bacteriocin, named serracin P, was purified from an induced culture supernatant of S. plymithicum J7. It was found to be the only compound involved in the antibacterial activity against sensitive strains. The N-terminal amino acid sequence analysis of the two major subunits (23 and 43 kDa) of serracin P revealed high homology with the Fels-2 prophage of Salmonella enterica, the coliphages P2 and 168, the {phi}CTX prophage of Pseudomonas aeruginosa, and a prophage of Yersinia pestis. This strongly suggests a common ancestry for serracin P and these bacteriophages.

* Corresponding author. Mailing address: Centre Wallon de Biologie Industrielle, University of Liege, BE-40, Sart Tilman, 4000 Liege, Belgium. Phone: 32 43 66 28 61. Fax: 32 43 66 28 62. E-mail: a_jabrane{at}hotmail.com.

Applied and Environmental Microbiology, November 2002, p. 5704-5710, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5704-5710.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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