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Applied and Environmental Microbiology, November 2002, p. 5765-5768, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5765-5768.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Transcript Analysis of Genes Encoding a Family 61 Endoglucanase and a Putative Membrane-Anchored Family 9 Glycosyl Hydrolase from Phanerochaete chrysosporium

Amber Vanden Wymelenberg,1 Stuart Denman,2,{dagger} Diane Dietrich,3 Jennifer Bassett,1,{ddagger} Xiaochun Yu,4 Rajai Atalla,3,4 Paul Predki,5,3 Ulla Rudsander,2 Tuula T. Teeri,2 and Daniel Cullen1,3*

Department of Bacteriology,1 Department of Chemical Engineering, University of Wisconsin—Madison, Madison, Wisconsin 53706,4 Department of Biotechnology, Royal Institute of Technology, Stockholm Centre for Physics, Astronomy, and Biotechnology, SE-106 91 Stockholm, Sweden,2 USDA Forest Products Laboratory, Madison, Wisconsin 53705,3 U.S. Department of Energy, Joint Genome Institute, Walnut Creek, California 945985

Received 13 May 2002/ Accepted 19 August 2002

Phanerochaete chrysosporium cellulase genes were cloned and characterized. The cel61A product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9A product revealed similarities to Thermobifida fusca Cel9A (E4), an enzyme with both endo- and exocellulase characteristics. The fungal Cel9A is apparently a membrane-bound protein, which is very unusual for microbial cellulases. Transcript levels of both genes were substantially higher in cellulose-grown cultures than in glucose-grown cultures. These results show that P. chrysosporium possesses a wide array of conventional and unconventional cellulase genes.

* Corresponding author. Mailing address: Forest Products Laboratory, One Gifford Pinchot Dr., Madison, WI 53705. Phone: (608) 231-9468. Fax: (608) 231-9262. E-mail: dcullen{at}facstaff.wisc.edu.

{dagger} Present address: CSIRO Livestock Industries, Indooroopilly, Qld 4068, Australia.

{ddagger} Present address: Department of Biomedical Engineering, University of Wisconsin—Madison, Madison, WI 53706.

§ Present address: Protometrix, Inc., Guilford, CT 06437.

Applied and Environmental Microbiology, November 2002, p. 5765-5768, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5765-5768.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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