This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fach, P.
Right arrow Articles by Broussolle, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fach, P.
Right arrow Articles by Broussolle, V.
Agricola
Right arrow Articles by Fach, P.
Right arrow Articles by Broussolle, V.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2002, p. 5870-5876, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5870-5876.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection by PCR-Enzyme-Linked Immunosorbent Assay of Clostridium botulinum in Fish and Environmental Samples from a Coastal Area in Northern France

Patrick Fach,1* Sylvie Perelle,1 Françoise Dilasser,1 Joël Grout,1 Claire Dargaignaratz,2 Lucien Botella,2 Jean-Marie Gourreau,3 Frédéric Carlin,2 Michel R. Popoff,4 and Véronique Broussolle2

Laboratoire d'Etudes et de Recherches sur l'Hygiène et la Qualité des Aliments (LERHQA), Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité ATB, 94700 Maisons-Alfort,1 Institut National de la Recherche Agronomique (INRA), UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, 84914 Avignon cedex 9,2 Laboratoire d'Etudes et de Recherches en Pathologie Animale et Zoonoses (LERPAZ), AFSSA, 94703 Maisons-Alfort,3 Institut Pasteur, Centre National de Référence des Anaérobies, 75015 Paris, France4

Received 20 February 2002/ Accepted 24 August 2002

The prevalence of Clostridium botulinum types A, B, E, and F was determined in 214 fresh fish and environmental samples collected in Northern France. A newly developed PCR-enzyme-linked immunosorbent assay (ELISA) used in this survey detected more than 80% of samples inoculated with fewer than 10 C. botulinum spores per 25 g and 100% of samples inoculated with more than 30 C. botulinum spores per 25 g. The percent agreement between PCR-ELISA and mouse bioassay was 88.9%, and PCR-ELISA detected more positive samples than the mouse bioassay did. The prevalence of C. botulinum in seawater fish and sediment was 16.6 and 4%, respectively, corresponding to 3.5 to 7 and 1 to 2 C. botulinum most-probable-number counts, respectively, and is in the low range of C. botulinum contamination reported elsewhere. The toxin type identification of the 31 naturally contaminated samples was 71% type B, 22.5% type A, and 9.6% type E. Type F was not detected. The high prevalence of C. botulinum type B in fish samples is relatively unusual compared with the high prevalence of C. botulinum type E reported in many worldwide and northern European surveys. However, fish processing and fish preparation in France have not been identified as a significant hazard for human type B botulism.

* Corresponding author. Mailing address: Laboratoire d'Etudes et de Recherches sur l'Hygiène et la Qualité des Aliments (LERHQA), Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité ATB, 1-5 rue de Belfort, 94700 Maisons-Alfort, France. Phone: 33 (0) 1 43 76 30 99. Fax: 33 (0) 1 43 76 26 30. E-mail: p.fach{at}afssa.fr.

Applied and Environmental Microbiology, December 2002, p. 5870-5876, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5870-5876.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • De Medici, D., Anniballi, F., Wyatt, G. M., Lindstrom, M., Messelhausser, U., Aldus, C. F., Delibato, E., Korkeala, H., Peck, M. W., Fenicia, L. (2009). Multiplex PCR for Detection of Botulinum Neurotoxin-Producing Clostridia in Clinical, Food, and Environmental Samples. Appl. Environ. Microbiol. 75: 6457-6461 [Abstract] [Full Text]  
  • Macdonald, T. E., Helma, C. H., Ticknor, L. O., Jackson, P. J., Okinaka, R. T., Smith, L. A., Smith, T. J., Hill, K. K. (2008). Differentiation of Clostridium botulinum Serotype A Strains by Multiple-Locus Variable-Number Tandem-Repeat Analysis. Appl. Environ. Microbiol. 74: 875-882 [Abstract] [Full Text]  
  • Fenicia, L., Anniballi, F., De Medici, D., Delibato, E., Aureli, P. (2007). SYBR Green Real-Time PCR Method To Detect Clostridium botulinum Type A. Appl. Environ. Microbiol. 73: 2891-2896 [Abstract] [Full Text]  
  • Sharma, S. K., Ferreira, Joseph. L., Eblen, B. S., Whiting, R. C. (2006). Detection of Type A, B, E, and F Clostridium botulinum Neurotoxins in Foods by Using an Amplified Enzyme-Linked Immunosorbent Assay with Digoxigenin-Labeled Antibodies. Appl. Environ. Microbiol. 72: 1231-1238 [Abstract] [Full Text]  
  • Nol, P., Williamson, J. L., Rocke, T. E., Yuill, T. M. (2004). Detection of Clostridium botulinum Type C Cells in the Gastrointestinal Tracts of Mozambique Tilapia (Oreochromis mossambicus) by Polymerase Chain Reaction. J Wildl Dis 40: 749-753 [Abstract] [Full Text]  
  • Nol, P., Rocke, T. E., Gross, K., Yuill, T. M. (2004). PREVALENCE OF NEUROTOXIC CLOSTRIDIUM BOTULINUM TYPE C IN THE GASTROINTESTINAL TRACTS OF TILAPIA (OREOCHROMIS MOSSAMBICUS) IN THE SALTON SEA. J Wildl Dis 40: 414-419 [Abstract] [Full Text]  
  • Hoorfar, J., Malorny, B., Abdulmawjood, A., Cook, N., Wagner, M., Fach, P. (2004). Practical Considerations in Design of Internal Amplification Controls for Diagnostic PCR Assays. J. Clin. Microbiol. 42: 1863-1868 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»