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Applied and Environmental Microbiology, December 2002, p. 5877-5881, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5877-5881.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of an Iron-Regulated, Hemin-Binding Outer Membrane Protein in Sinorhizobium meliloti

Federico Battistoni,1,2 Raúl Platero,1 Rosario Duran,2 Carlos Cerveñansky,2 Julio Battistoni,3 Alicia Arias,1 and Elena Fabiano1*

Laboratorio de Ecología Microbiana,1 Unidad de Bioquímica Analítica, Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE-MEC), Montevideo 11600,2 Laboratorio de Productos Inmuno-Biotecnológicos, Unidades Asociadas a Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay3

Received 23 May 2002/ Accepted 29 August 2002

Rhizobia are soil bacteria that are able to establish symbiotic associations with leguminous hosts. In iron-limited environments these bacteria can use iron present in heme or heme compounds (hemoglobin, leghemoglobin). Here we report the presence in Sinorhizobium meliloti of an iron-regulated outer membrane protein that is able to bind hemin but not hemoglobin. Protein assignment was done by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Tryptic peptides correlated with the mass measurements obtained accounted for 54% of the translated sequence of a putative heme receptor gene present in the chromosome of S. meliloti 1021. The results which we obtained suggest that this protein (designated ShmR for Sinorhizobium heme receptor) is involved in high-affinity heme-mediated iron transport.

* Corresponding author. Mailing address: Laboratorio de Ecologia Microbiana, IIBCE, Av. Italia 3318, Montevideo 11600, Uruguay. Phone: 598 (2) 4871616, ext. 146. Fax: 598 (2) 4875548. E-mail: efabiano{at}iibce.edu.uy.

Applied and Environmental Microbiology, December 2002, p. 5877-5881, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5877-5881.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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