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Applied and Environmental Microbiology, December 2002, p. 5973-5980, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5973-5980.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Arthrobacter aurescens TC1 Metabolizes Diverse s-Triazine Ring Compounds

Lisa C. Strong,1,2 Charlotte Rosendahl,1 Gilbert Johnson,1,2 Michael J. Sadowsky,2,3 and Lawrence P. Wackett1,2*

Department of Biochemistry, Molecular Biology and Biophysics,1 Biotechnology Institute,2 Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota 551083

Received 5 June 2002/ Accepted 24 September 2002

Arthrobacter aurescens strain TC1 was isolated without enrichment by plating atrazine-contaminated soil directly onto atrazine-clearing plates. A. aurescens TC1 grew in liquid medium with atrazine as the sole source of nitrogen, carbon, and energy, consuming up to 3,000 mg of atrazine per liter. A. aurescens TC1 is metabolically diverse and grew on a wider range of s-triazine compounds than any bacterium previously characterized. The 23 s-triazine substrates serving as the sole nitrogen source included the herbicides ametryn, atratone, cyanazine, prometryn, and simazine. Moreover, atrazine substrate analogs containing fluorine, mercaptan, and cyano groups in place of the chlorine substituent were also growth substrates. Analogs containing hydrogen, azido, and amino functionalities in place of chlorine were not growth substrates. A. aurescens TC1 also metabolized compounds containing chlorine plus N-ethyl, N-propyl, N-butyl, N-s-butyl, N-isobutyl, or N-t-butyl substituents on the s-triazine ring. Atrazine was metabolized to alkylamines and cyanuric acid, the latter accumulating stoichiometrically. Ethylamine and isopropylamine each served as the source of carbon and nitrogen for growth. PCR experiments identified genes with high sequence identity to atzB and atzC, but not to atzA, from Pseudomonas sp. strain ADP.

* Corresponding author. Mailing address: Department of Biochemistry, Molecular Biology, and Biophysics and Biotechnology Institute, 140 Gortner Lab, 1479 Gortner Ave., University of Minnesota, St. Paul, MN 55108. Phone: (612) 625-3785. Fax: (612) 625-1700. E-mail: wackett{at}biosci.cbs.umn.edu.

Applied and Environmental Microbiology, December 2002, p. 5973-5980, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5973-5980.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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