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Applied and Environmental Microbiology, December 2002, p. 6094-6105, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6094-6105.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Impact of Protozoan Grazing on Bacterial Community Structure in Soil Microcosms

Regin Rønn,1,2* Allison E. McCaig,1 Bryan S. Griffiths,3 and James I. Prosser1

Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD,1 Soil-Plant Dynamics Group, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom,3 Department of Evolutionary Biology, Zoological Institute, University of Copenhagen, DK-2100 Copenhagen, Denmark2

Received 10 June 2002/ Accepted 16 September 2002

The influence of grazing by a mixed assemblage of soil protozoa (seven flagellates and one amoeba) on bacterial community structure was studied in soil microcosms amended with a particulate resource (sterile wheat roots) or a soluble resource (a solution of various organic compounds). Sterilized soil was reinoculated with mixed soil bacteria (obtained by filtering and dilution) or with bacteria and protozoa. Denaturing gradient gel electrophoresis (DGGE) of PCR amplifications of 16S rRNA gene fragments, as well as community level physiological profiling (Biolog plates), suggested that the mixed protozoan community had significant effects on the bacterial community structure. Excising and sequencing of bands from the DGGE gels indicated that high-G+C gram-positive bacteria closely related to Arthrobacter spp. were favored by grazing, whereas the excised bands that decreased in intensity were related to gram-negative bacteria. The percentages of intensity found in bands related to high G+C gram positives increased from 4.5 and 12.6% in the ungrazed microcosms amended with roots and nutrient solution, respectively, to 19.3 and 32.9% in the grazed microcosms. Protozoa reduced the average bacterial cell size in microcosms amended with nutrient solution but not in the treatment amended with roots. Hence, size-selective feeding may explain some but not all of the changes in bacterial community structure. Five different protozoan isolates (Acanthamoeba sp., two species of Cercomonas, Thaumatomonas sp., and Spumella sp.) had different effects on the bacterial communities. This suggests that the composition of protozoan communities is important for the effect of protozoan grazing on bacterial communities.

* Corresponding author. Mailing address: Department of Evolutionary Biology, Zoological Institute, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark. Phone (45) 35321314. Fax. (45) 35321300. E-mail: rronn{at}zi.ku.dk.

Applied and Environmental Microbiology, December 2002, p. 6094-6105, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6094-6105.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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