This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hiett, K. L
Right arrow Articles by Ladely, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hiett, K. L
Right arrow Articles by Ladely, S.
Agricola
Right arrow Articles by Hiett, K. L
Right arrow Articles by Ladely, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2002, p. 6220-6236, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6220-6236.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Subtype Analyses of Campylobacter spp. from Arkansas and California Poultry Operations

K. L Hiett,1* N. J. Stern,1 P. Fedorka-Cray,2 N. A. Cox,1 M. T. Musgrove,1 and S. Ladely2

Poultry Microbiological Safety Research Unit,1 Antimicrobial Resistance Research Unit, USDA Agricultural Research Service, Athens, Georgia2

Received 22 March 2002/ Accepted 23 August 2002

Campylobacter isolates from diverse samples within broiler production and processing environments were typed by using flaA short variable region DNA sequence analysis. Sixteen flocks from four different farms representing two broiler producers in Arkansas and California were analyzed. Fourteen of the flocks (87.5%) were Campylobacter-positive; two remained negative throughout the 6-week rearing period. In general, multiple clones were present within a flock. Additionally, clones found within a flock were also present on the final product, although the diversity of Campylobacter spp. on the final product appeared to be reduced relative to that observed within the flock. Comparison of clones between flocks on the same farm revealed that some clones of Campylobacter persisted in multiple flocks. Furthermore, some clones were identified across the two farms that were under the same management. In two sampling periods, environmental isolates were positive for Campylobacter prior to flock shedding. Environmental samples associated with five additional flocks were positive for Campylobacter concomitantly with recovery of Campylobacter from the birds. Analysis of the environmental isolates that were positive prior to flock shedding demonstrated that in some instances the environmental isolates possessed genotypes identical to those of isolates originating from the flock, while in other cases the environmental isolates possessed genotypes that were distantly related to isolates obtained from the flock. Analyses of environmental isolates that tested positive concurrently with the positive isolates from the flocks demonstrated varied results; in some instances the environmental isolates possessed genotypes identical to those of isolates originating from the flock, while in other cases the environmental isolates possessed genotypes that were distantly related to isolates obtained from the flock. These data suggest that the external environment may contribute to Campylobacter contamination during poultry production and processing. However, environmental contamination with Campylobacter does not appear to be the sole contributing factor.

* Corresponding author. Mailing address: USDA Agricultural Research Service, Poultry Microbiological Safety Research Unit, Russell Research Center, P.O. Box 5677, Athens, GA 30604-5677. Phone: (706) 546-3593. Fax: (706) 546-3772. E-mail: khiett{at}saa.ars.usda.gov.

Applied and Environmental Microbiology, December 2002, p. 6220-6236, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6220-6236.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Habib, I., Miller, W. G., Uyttendaele, M., Houf, K., De Zutter, L. (2009). Clonal Population Structure and Antimicrobial Resistance of Campylobacter jejuni in Chicken Meat from Belgium. Appl. Environ. Microbiol. 75: 4264-4272 [Abstract] [Full Text]  
  • Forbes, K. J., Gormley, F. J., Dallas, J. F., Labovitiadi, O., MacRae, M., Owen, R. J., Richardson, J., Strachan, N. J. C., Cowden, J. M., Ogden, I. D., McGuigan, C. C. (2009). Campylobacter Immunity and Coinfection following a Large Outbreak in a Farming Community. J. Clin. Microbiol. 47: 111-116 [Abstract] [Full Text]  
  • Callicott, K. A., Harthardottir, H., Georgsson, F., Reiersen, J., Frithriksdottir, V., Gunnarsson, E., Michel, P., Bisaillon, J.-R., Kristinsson, K. G., Briem, H., Hiett, K. L., Needleman, D. S., Stern, N. J. (2008). Broiler Campylobacter Contamination and Human Campylobacteriosis in Iceland. Appl. Environ. Microbiol. 74: 6483-6494 [Abstract] [Full Text]  
  • De Cesare, A., Parisi, A., Bondioli, V., Normanno, G., Manfreda, G. (2008). Genotypic and Phenotypic Diversity Within Three Campylobacter Populations Isolated from Broiler Ceca and Carcasses. Poult. Sci. 87: 2152-2159 [Abstract] [Full Text]  
  • Ridley, A. M., Allen, V. M., Sharma, M., Harris, J. A., Newell, D. G. (2008). Real-Time PCR Approach for Detection of Environmental Sources of Campylobacter Strains Colonizing Broiler Flocks. Appl. Environ. Microbiol. 74: 2492-2504 [Abstract] [Full Text]  
  • Elvers, K. T., Helps, C. R., Wassenaar, T. M., Allen, V. M., Newell, D. G. (2008). Development of a Strain-Specific Molecular Method for Quantitating Individual Campylobacter Strains in Mixed Populations. Appl. Environ. Microbiol. 74: 2321-2331 [Abstract] [Full Text]  
  • Conlan, A. J.K, Coward, C., Grant, A. J, Maskell, D. J, Gog, J. R (2007). Campylobacter jejuni colonization and transmission in broiler chickens: a modelling perspective. J R Soc Interface 4: 819-829 [Abstract] [Full Text]  
  • Ogden, I. D., MacRae, M., Johnston, M., Strachan, N. J. C., Cody, A. J., Dingle, K. E., Newell, D. G. (2007). Use of Multilocus Sequence Typing To Investigate the Association between the Presence of Campylobacter spp. in Broiler Drinking Water and Campylobacter Colonization in Broilers. Appl. Environ. Microbiol. 73: 5125-5129 [Abstract] [Full Text]  
  • Vereen, E. Jr., Lowrance, R. R., Cole, D. J., Lipp, E. K. (2007). Distribution and Ecology of Campylobacters in Coastal Plain Streams (Georgia, United States of America). Appl. Environ. Microbiol. 73: 1395-1403 [Abstract] [Full Text]  
  • Bull, S. A., Allen, V. M., Domingue, G., Jorgensen, F., Frost, J. A., Ure, R., Whyte, R., Tinker, D., Corry, J. E. L., Gillard-King, J., Humphrey, T. J. (2006). Sources of Campylobacter spp. Colonizing Housed Broiler Flocks during Rearing. Appl. Environ. Microbiol. 72: 645-652 [Abstract] [Full Text]  
  • Meerburg, B. G., Jacobs-Reitsma, W. F., Wagenaar, J. A., Kijlstra, A. (2006). Presence of Salmonella and Campylobacter spp. in Wild Small Mammals on Organic Farms. Appl. Environ. Microbiol. 72: 960-962 [Abstract] [Full Text]  
  • Meinersmann, R. J., Phillips, R. W., Hiett, K. L., Fedorka-Cray, P. (2005). Differentiation of Campylobacter Populations as Demonstrated by Flagellin Short Variable Region Sequences. Appl. Environ. Microbiol. 71: 6368-6374 [Abstract] [Full Text]  
  • Connerton, P. L., Loc Carrillo, C. M., Swift, C., Dillon, E., Scott, A., Rees, C. E. D., Dodd, C. E. R., Frost, J., Connerton, I. F. (2004). Longitudinal Study of Campylobacter jejuni Bacteriophages and Their Hosts from Broiler Chickens. Appl. Environ. Microbiol. 70: 3877-3883 [Abstract] [Full Text]  
  • Sails, A. D., Swaminathan, B., Fields, P. I. (2003). Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni. J. Clin. Microbiol. 41: 4733-4739 [Abstract] [Full Text]  
  • Newell, D. G., Fearnley, C. (2003). Sources of Campylobacter Colonization in Broiler Chickens. Appl. Environ. Microbiol. 69: 4343-4351 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»