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Applied and Environmental Microbiology, December 2002, p. 6332-6342, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6332-6342.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effect of Different NADH Oxidase Levels on Glucose Metabolism by Lactococcus lactis: Kinetics of Intracellular Metabolite Pools Determined by In Vivo Nuclear Magnetic Resonance

Ana Rute Neves,1 Ana Ramos,1 Helena Costa,1 Iris I. van Swam,2 Jeroen Hugenholtz,2 Michiel Kleerebezem,2 Willem de Vos,2 and Helena Santos1*

Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa and Instituto de Biologia Experimental e Tecnológica, 2780-156 Oeiras, Portugal,1 Wageningen Centre for Food Sciences Food Research/NIZO, 6710 BA Ede, The Netherlands2

Received 13 May 2002/ Accepted 5 September 2002

Three isogenic strains of Lactococcus lactis with different levels of H2O-forming NADH oxidase activity were used to study the effect of oxygen on glucose metabolism: the parent strain L. lactis MG1363, a NOX- strain harboring a deletion of the gene coding for H2O-forming NADH oxidase, and a NOX+ strain with the NADH oxidase activity enhanced by about 100-fold. A comprehensive description of the metabolic events was obtained by using 13C nuclear magnetic resonance in vivo. The most noticeable results of this study are as follows: (i) under aerobic conditions the level of fructose 1,6-bisphosphate [Fru(1,6)P2] was lower than the level under anaerobic conditions, and the rate of Fru(1,6)P2 depletion was very high; (ii) the levels of 3-phosphoglycerate and phosphoenolpyruvate were considerably enhanced under aerobic conditions and significantly lower in the NOX- strain; and (iii) the glycolytic flux decreased in the presence of saturating levels of oxygen, but it was not altered in response to changes in the NADH oxidase activity. In particular, the observation that the glycolytic flux was not enhanced in the NOX+ strain indicated that glycolytic flux was not primarily determined by the level of NADH in the cell. The patterns of end products were identical for the NOX- and parent strains; in the NOX+ strain the carbon flux was diverted to the production of {alpha}-acetolactate-derived compounds, and at a low pH this strain produced diacetyl at concentrations up to 1.6 mM. The data were integrated with the goal of identifying the main regulatory aspects of glucose metabolism in the presence of oxygen.

* Corresponding author. Mailing address: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua Da Quinta Grande, 6, Apt. 127, 2780-156 Oeiras, Portugal. Phone: 351-214469828. Fax: 351-214428766. E-mail: santos{at}itqb.unl.pt.

Applied and Environmental Microbiology, December 2002, p. 6332-6342, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6332-6342.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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