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Applied and Environmental Microbiology, December 2002, p. 6410-6415, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6410-6415.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Existence of a True Phosphofructokinase in Bacillus sphaericus: Cloning and Sequencing of the pfk Gene

Alejandro F. Alice,1 Gaspar Pérez-Martínez,2 and Carmen Sánchez-Rivas1*

Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina,1 Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Paterna, Valencia, Spain2

Received 6 June 2002/ Accepted 26 August 2002

Some strains of Bacillus sphaericus are entomopathogenic to mosquito larvae, which transmit diseases, such as filariasis and malaria, affecting millions of people worldwide. This species is unable to use hexoses and pentoses as unique carbon sources, which was proposed to be due to the lack of glycolytic enzymes, such as 6-phosphofructokinase (PFK). In this study, PFK activity was detected and the pfk gene was cloned and sequenced. Furthermore, this gene was shown to be present in strains belonging to all the homology groups of this heterogeneous species, in which PFK activity was also detected. A careful sequence analysis revealed the conservation of different catalytic and regulatory residues, as well as the enzyme's phylogenetic affiliation with the family of allosteric ATP-PFK enzymes.

* Corresponding author. Mailing address: Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires (1428), Argentina. Phone: 54-11-45763300. Fax: 54-11-45763342. E-mail: sanchez{at}qb.fcen.uba.ar.

Applied and Environmental Microbiology, December 2002, p. 6410-6415, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6410-6415.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Han, B., Liu, H., Hu, X., Cai, Y., Zheng, D., Yuan, Z. (2007). Molecular Characterization of a Glucokinase with Broad Hexose Specificity from Bacillus sphaericus Strain C3-41. Appl. Environ. Microbiol. 73: 3581-3586 [Abstract] [Full Text]  
  • Alice, A. F., Perez-Martinez, G., Sanchez-Rivas, C. (2003). Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus. Microbiology 149: 1687-1698 [Abstract] [Full Text]  


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