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Applied and Environmental Microbiology, December 2002, p. 6421-6424, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6421-6424.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium

Elena Barbieri,1 Giulia Riccioni,1 Anna Pisano,1 Davide Sisti,2 Sabrina Zeppa,1 Deborah Agostini,1 and Vilberto Stocchi1*

"Giorgio Fornaini" Institute of Biochemistry,1 Institute of Botany, University of Urbino, 61029 Urbino, Pesaro-Urbino, Italy2

Received 12 April 2002/ Accepted 29 August 2002

An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 106 cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.

* Corresponding author. Mailing address: Istituto di Chimica Biologica "Giorgio Fornaini," University of Urbino, Via A. Saffi, 2, 61029 Urbino (PU), Italy. Phone: 39 0722 305262. Fax: 39 0722 320188. E-mail: v.stocchi{at}uniurb.it.

Applied and Environmental Microbiology, December 2002, p. 6421-6424, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6421-6424.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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