This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Barbieri, E. Articles by Stocchi, V. Search for Related Content PubMed PubMed Citation Articles by Barbieri, E. Articles by Stocchi, V. Agricola Articles by Barbieri, E. Articles by Stocchi, V.

Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium

"Giorgio Fornaini" Institute of Biochemistry,¹ Institute of Botany, University of Urbino, 61029 Urbino, Pesaro-Urbino, Italy²

Received 12 April 2002/ Accepted 29 August 2002

An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10⁶ cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.