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Applied and Environmental Microbiology, December 2002, p. 6429-6434, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6429-6434.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Identification, Differentiation, and Proposed New Taxonomic Classification of Bifidobacterium lactis

Marco Ventura and Ralf Zink*

Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland

Received 22 April 2002/ Accepted 16 September 2002

Identification of Bifidobacterium lactis and Bifidobacterium animalis is problematic because of phenotypic and genetic homogeneities and has raised the question of whether they belong to one unique taxon. Analysis of the 16S-23S internally transcribed spacer region of B. lactis DSM10140T, B. animalis ATCC 25527T, and six potential B. lactis strains suggested two distinct clusters. Two specific 16S-23S spacer rRNA gene-targeted primers have been developed for specific detection of B. animalis. All of the molecular techniques used (B. lactis or B. animalis PCR primers, enterobacterial repetitive intergenic consensus PCR) demonstrated that B. lactis and B. animalis form two main groups and suggest a revision of the strains assigned to B. animalis. We propose that B. lactis should be separated from B. animalis at the subspecies level.

* Corresponding author. Mailing address: Nestlé Research Center, Route du Jourat 57, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland. Phone: 41-21-785-8901. Fax: 41-21-785-8925. E-mail: ralf.zink{at}rdls.nestle.com.

Applied and Environmental Microbiology, December 2002, p. 6429-6434, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6429-6434.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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