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Applied and Environmental Microbiology, December 2002, p. 6435-6438, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6435-6438.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Incorporation of DNA and Protein Precursors into Macromolecules by Bacteria at -15oC

Brent C. Christner*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210-1292

Received 24 June 2002/ Accepted 30 August 2002

DNA and protein precursors were incorporated into trichloroacetic acid-precipitated material by bacterial cell suspensions during incubation for 50 to 100 days at -15oC. Incorporation did not occur at -70oC and was inhibited by antibiotics. The results demonstrate that bacteria can perform macromolecular synthesis under conditions that mimic entrapment in glacial ice.


* Present address: Land Resources & Environmental Sciences, Montana State University, 334 Leon Johnson Hall, Bozeman, MT 59717. Phone: (406) 994-2733. Fax: (406) 994-5863. E-mail: bchristner{at}montana.edu.


Applied and Environmental Microbiology, December 2002, p. 6435-6438, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6435-6438.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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