This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Christner, B. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christner, B. C.
Agricola
Right arrow Articles by Christner, B. C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2002, p. 6435-6438, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6435-6438.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Incorporation of DNA and Protein Precursors into Macromolecules by Bacteria at -15oC

Brent C. Christner*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210-1292

Received 24 June 2002/ Accepted 30 August 2002

DNA and protein precursors were incorporated into trichloroacetic acid-precipitated material by bacterial cell suspensions during incubation for 50 to 100 days at -15oC. Incorporation did not occur at -70oC and was inhibited by antibiotics. The results demonstrate that bacteria can perform macromolecular synthesis under conditions that mimic entrapment in glacial ice.


* Present address: Land Resources & Environmental Sciences, Montana State University, 334 Leon Johnson Hall, Bozeman, MT 59717. Phone: (406) 994-2733. Fax: (406) 994-5863. E-mail: bchristner{at}montana.edu.


Applied and Environmental Microbiology, December 2002, p. 6435-6438, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6435-6438.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Bergholz, P. W., Bakermans, C., Tiedje, J. M. (2009). Psychrobacter arcticus 273-4 Uses Resource Efficiency and Molecular Motion Adaptations for Subzero Temperature Growth. J. Bacteriol. 191: 2340-2352 [Abstract] [Full Text]  
  • Amato, P., Christner, B. C. (2009). Energy Metabolism Response to Low-Temperature and Frozen Conditions in Psychrobacter cryohalolentis. Appl. Environ. Microbiol. 75: 711-718 [Abstract] [Full Text]  
  • Skidmore, M., Anderson, S. P., Sharp, M., Foght, J., Lanoil, B. D. (2005). Comparison of Microbial Community Compositions of Two Subglacial Environments Reveals a Possible Role for Microbes in Chemical Weathering Processes. Appl. Environ. Microbiol. 71: 6986-6997 [Abstract] [Full Text]  
  • Juck, D. F., Whissell, G., Steven, B., Pollard, W., McKay, C. P., Greer, C. W., Whyte, L. G. (2005). Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic. Appl. Environ. Microbiol. 71: 1035-1041 [Abstract] [Full Text]  
  • Price, P. B., Sowers, T. (2004). Temperature dependence of metabolic rates for microbial growth, maintenance, and survival. Proc. Natl. Acad. Sci. USA 101: 4631-4636 [Abstract] [Full Text]  
  • Junge, K., Eicken, H., Deming, J. W. (2004). Bacterial Activity at -2 to -20{degrees}C in Arctic Wintertime Sea Ice. Appl. Environ. Microbiol. 70: 550-557 [Abstract] [Full Text]  
  • Warren, S. G., Hudson, S. R., Carpenter, E. J., Capone, D. G. (2003). Bacterial Activity in South Pole Snow Is Questionable. Appl. Environ. Microbiol. 69: 6340-6341 [Full Text]  
  • Junge, K., Eicken, H., Deming, J. W. (2003). Motility of Colwellia psychrerythraea Strain 34H at Subzero Temperatures. Appl. Environ. Microbiol. 69: 4282-4284 [Abstract] [Full Text]