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Applied and Environmental Microbiology, December 2002, p. 6451-6456, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6451-6456.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector

Franck Biet,1* Yves Cenatiempo,1 and Christophe Fremaux2

Institut de Biologie Moléculaire et d'Ingénierie Génétique, Equipe d’Accueil 2224, Université de Poitiers, 86022 Poitiers Cedex,1 Rhodia Food, Zone d’Activités de Buxières, 86220 Dangé Saint-Romain, France2

Received 29 August 2002/ Accepted 5 September 2002

A 2,665-bp cryptic plasmid, pTXL1, isolated from Leuconostoc mesenteroides subsp. mesenteroides Y110 was identified. This plasmid harbors a replicon localized on a 1,300-bp fragment. Two observations suggested that pTXL1 does not belong to rolling-circle replication (RCR)-type plasmids and most likely replicates via a theta mechanism. These hypotheses are supported by the observation that no detectable single-stranded intermediate was found for the replicon and that, unlike in RCR-type plasmids, the pTXL1 replicon sequence lacks an open reading frame encoding a replicase. The small-sized pTXL1 plasmid is stable and, according to its origin, can be considered in the "generally recognized as safe" category. Its ability to replicate in several lactic acid bacteria was exploited to develop a vector producing mesentericin Y105, a class II anti-Listeria bacteriocin. With this new vector, a recombinant industrial Leuconostoc cremoris strain able to produce mesentericin Y105 was constructed.

* Corresponding author. Present address: LBE/IBL Institut Pasteur de Lille, 1 rue du Prof. Calmette, B.P. 245, 59019 Lille Cedex, France. Phone: 33-3-2087 1195. Fax: 33-3-2087 1192. E-mail: franck.biet{at}pasteur-lille.fr.

Applied and Environmental Microbiology, December 2002, p. 6451-6456, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6451-6456.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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