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Applied and Environmental Microbiology, December 2002, p. 6462-6465, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6462-6465.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Disruption of narH, narJ, and moaE Inhibits Heterotrophic Nitrification in Pseudomonas Strain M19

D. R. Nemergut and S. K. Schmidt*

Department of Environmental, Population and Organismic Biology, University of Colorado, Boulder, Colorado 80309

Received 25 April 2002/ Accepted 6 September 2002

Interruptions in three nitrate reductase-related genes, narH, narJ, and moaE, inhibited heterotrophic nitrification in Pseudomonas strain M19. No nitrate was detected in the medium, and nitrification proceeded in the presence of a nitrate reductase inhibitor. Heterotrophic nitrification was greatly stimulated by the addition of nitrate.

* Corresponding author. Mailing address: Department of Environmental, Population and Organismic Biology, University of Colorado, Campus Box 334, Boulder, CO 80309. Phone: (303) 492-6248. Fax: (303) 492-8699. E-mail: schmidts{at}spot.colorado.edu.

Applied and Environmental Microbiology, December 2002, p. 6462-6465, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6462-6465.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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