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Enumeration of Legionella CFU by Colony Hybridization Using Specific DNA Probes

Technology and Development Laboratory, Kurita Water Industries Ltd., Kanagawa 243-0124,¹ Kurita Sogo Service Co., Ltd., Kanagawa 234-0124,² St. Marianna University School of Medicine, Kanagawa 216-8511,³ Gifu University School of Medicine, Gifu 500,⁴ Department of Bacteriology, Juntendo University, Tokyo 113-8421, Japan⁵

Received 16 October 2001/ Accepted 17 September 2002

Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.

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