This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Satoh, W.
Right arrow Articles by Hiramatsu, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Satoh, W.
Right arrow Articles by Hiramatsu, K.
Agricola
Right arrow Articles by Satoh, W.
Right arrow Articles by Hiramatsu, K.

 Previous Article

Applied and Environmental Microbiology, December 2002, p. 6466-6470, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6466-6470.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Enumeration of Legionella CFU by Colony Hybridization Using Specific DNA Probes

Wakako Satoh,1* Mayumi Nakata,2 Hiroyuki Yamamoto,3 Takayuki Ezaki,4 and Keiichi Hiramatsu5

Technology and Development Laboratory, Kurita Water Industries Ltd., Kanagawa 243-0124,1 Kurita Sogo Service Co., Ltd., Kanagawa 234-0124,2 St. Marianna University School of Medicine, Kanagawa 216-8511,3 Gifu University School of Medicine, Gifu 500,4 Department of Bacteriology, Juntendo University, Tokyo 113-8421, Japan5

Received 16 October 2001/ Accepted 17 September 2002

Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.

* Corresponding author. Mailing address: Technology and Development Laboratory, Kurita Water Industries Ltd., 7-1, Wakamiya Morinosato, Atsugi-city, Kanagawa 243-0124, Japan. Phone: 81 046 270 2103. Fax: 81 046 270 2149. E-mail: wakako.Satoh{at}kurita.co.jp.

Applied and Environmental Microbiology, December 2002, p. 6466-6470, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6466-6470.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Elvers, K. T., Helps, C. R., Wassenaar, T. M., Allen, V. M., Newell, D. G. (2008). Development of a Strain-Specific Molecular Method for Quantitating Individual Campylobacter Strains in Mixed Populations. Appl. Environ. Microbiol. 74: 2321-2331 [Abstract] [Full Text]  
  • Aurell, H., Catala, P., Farge, P., Wallet, F., Le Brun, M., Helbig, J. H., Jarraud, S., Lebaron, P. (2004). Rapid Detection and Enumeration of Legionella pneumophila in Hot Water Systems by Solid-Phase Cytometry. Appl. Environ. Microbiol. 70: 1651-1657 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»