This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scortichini, M.
Right arrow Articles by Di Prospero, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scortichini, M.
Right arrow Articles by Di Prospero, P.
Agricola
Right arrow Articles by Scortichini, M.
Right arrow Articles by Di Prospero, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2002, p. 476-484, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.476-484.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bacteria Associated with Hazelnut (Corylus avellana L.) Decline Are of Two Groups: Pseudomonas avellanae and Strains Resembling P. syringae pv. syringae

Marco Scortichini,{dagger},* Ugo Marchesi, Maria Pia Rossi, and Paola Di Prospero

Istituto Sperimentale per la Frutticoltura, Rome, Italy

Received 12 June 2001/ Accepted 9 October 2001

A total of 118 fluorescent pseudomonads associated with hazelnut decline, which has been occurring for many years in different areas of northern Greece and Italy, were assessed by performing a repetitive PCR analysis with enterobacterial repetitive intergenic consensus, box element, and repetive extragenic palindromic primer sets, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell protein extracts, a carbon compound utilization analysis, and an analysis to determine the presence of the syrB gene. A subset of 53 strains was also characterized by amplified 16S ribosomal DNA restriction analysis (ARDRA) by using nine restriction endonucleases. The virulence of 40 representative strains was assessed by using serial doses. The pathogenic specificities of the strains were also verified. ARDRA carried out with HinfI revealed two main groups of strains, groups A and B, which exhibited a level of similarity of 57%. The other eight restriction endonucleases used did not separate the strains. In addition, a cluster analysis performed by the unweighted pair group method using arithmetic averages after repetitive PCR and SDS-PAGE of protein extracts also revealed the same two groups. Furthermore, the differential utilization of some carbon compounds made it possible to differentiate the groups. Virulence assessment clearly indicated that the group A strains are very virulent, whereas the group B strains proved to be mildly virulent for hazelnut. Group A included the strains isolated in northern Greece and central Italy (i.e., the province of Viterbo); these strains do not have the syrB gene, are pathogenically restricted to Corylus avellana, and belong to Pseudomonas avellanae. Group B includes the other strains obtained from hazelnut cultivated in Piedmont, Campania, Latium, Sicily, and Sardinia. They represent a distinct taxon closely related to Pseudomonas syringae pv. syringae.

* Corresponding author. Mailing address: Istituto Sperimentale per la Frutticoltura, Via di Fioranello, 52 I-00040 Ciampino Aeroporto, Rome, Italy. Phone: 39 0679348147. Fax: 39 0679340158. E-mail: mscortichini{at}hotmail.com.

{dagger} M. Scortichini is a staff member of the Istituto Sperimentale per la Patologia Vegetale, Rome, temporarily assigned to Istituto Sperimentale per la Frutticoltura.

Applied and Environmental Microbiology, February 2002, p. 476-484, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.476-484.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wang, P. W., Morgan, R. L., Scortichini, M., Guttman, D. S. (2007). Convergent evolution of phytopathogenic pseudomonads onto hazelnut. Microbiology 153: 2067-2073 [Abstract] [Full Text]  
  • Scortichini, M., Natalini, E., Angelucci, L. (2003). Clonal population structure of Pseudomonas avellanae strains of different origin based on multilocus enzyme electrophoresis. Microbiology 149: 2891-2900 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»