This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hendrickson, E. R.
Right arrow Articles by Ebersole, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hendrickson, E. R.
Right arrow Articles by Ebersole, R. C.
Agricola
Right arrow Articles by Hendrickson, E. R.
Right arrow Articles by Ebersole, R. C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2002, p. 485-495, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.485-495.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe

Edwin R. Hendrickson,1* Jo Ann Payne,1 Roslyn M. Young,2 Mark G. Starr,1 Michael P. Perry,2 Stephen Fahnestock,2 David E. Ellis,3 and Richard C. Ebersole1

Corporate Center for Engineering Research, Central Research and Development, E. I. Du Pont de Nemours & Co., Glasgow Site, Newark, Delaware 19714,1 Biological SciencesEngineering, Central Research and Development, E. I. Du Pont de Nemours & Co., Wilmington, Delaware 19880,,2 Corporate Remediation Group, E. I. Du Pont de Nemours & Co., Wilmington, Delaware 198053

Received 8 June 2001/ Accepted 24 October 2001

The environmental distribution of Dehalococcoides group organisms and their association with chloroethene-contaminated sites were examined. Samples from 24 chloroethene-dechlorinating sites scattered throughout North America and Europe were tested for the presence of members of the Dehalococcoides group by using a PCR assay developed to detect Dehalococcoides 16S rRNA gene (rDNA) sequences. Sequences identified by sequence analysis as sequences of members of the Dehalococcoides group were detected at 21 sites. Full dechlorination of chloroethenes to ethene occurred at these sites. Dehalococcoides sequences were not detected in samples from three sites at which partial dechlorination of chloroethenes occurred, where dechlorination appeared to stop at 1,2-cis-dichloroethene. Phylogenetic analysis of the 16S rDNA amplicons confirmed that Dehalococcoides sequences formed a unique 16S rDNA group. These 16S rDNA sequences were divided into three subgroups based on specific base substitution patterns in variable regions 2 and 6 of the Dehalococcoides 16S rDNA sequence. Analyses also demonstrated that specific base substitution patterns were signature patterns. The specific base substitutions distinguished the three sequence subgroups phylogenetically. These results demonstrated that members of the Dehalococcoides group are widely distributed in nature and can be found in a variety of geological formations and in different climatic zones. Furthermore, the association of these organisms with full dechlorination of chloroethenes suggests that they are promising candidates for engineered bioremediation and may be important contributors to natural attenuation of chloroethenes.

* Corresponding author. Mailing address: E. I. Du Pont de Nemours & Co., Environmental and Microbiological Sciences and Engineering, Corporate Center for Engineering Research, Central Research & Development, P.O. Box 6101, Glasgow Site 300/318, Newark, DE 19714- 6101. Phone: (302) 366-6704. Fax: (302) 366-6607. E-mail: edwin.r.hendrickson{at}usa.dupont.com.

Applied and Environmental Microbiology, February 2002, p. 485-495, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.485-495.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moe, W. M., Yan, J., Nobre, M. F., da Costa, M. S., Rainey, F. A. (2009). Dehalogenimonas lykanthroporepellens gen. nov., sp. nov., a reductively dehalogenating bacterium isolated from chlorinated solvent-contaminated groundwater. Int. J. Syst. Evol. Microbiol. 59: 2692-2697 [Abstract] [Full Text]  
  • Tas, N., van Eekert, M. H. A., Schraa, G., Zhou, J., de Vos, W. M., Smidt, H. (2009). Tracking Functional Guilds: "Dehalococcoides" spp. in European River Basins Contaminated with Hexachlorobenzene. Appl. Environ. Microbiol. 75: 4696-4704 [Abstract] [Full Text]  
  • Grostern, A., Edwards, E. A. (2009). Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene. Appl. Environ. Microbiol. 75: 2684-2693 [Abstract] [Full Text]  
  • Lee, P. K. H., Macbeth, T. W., Sorenson, K. S. Jr., Deeb, R. A., Alvarez-Cohen, L. (2008). Quantifying Genes and Transcripts To Assess the In Situ Physiology of "Dehalococcoides" spp. in a Trichloroethene-Contaminated Groundwater Site. Appl. Environ. Microbiol. 74: 2728-2739 [Abstract] [Full Text]  
  • Nikolausz, M., Chatzinotas, A., Palatinszky, M., Imfeld, G., Martinez, P., Kastner, M. (2008). Single-Nucleotide Primer Extension Assay for Detection and Sequence Typing of "Dehalococcoides" spp.. Appl. Environ. Microbiol. 74: 300-304 [Abstract] [Full Text]  
  • Bedard, D. L., Ritalahti, K. M., Loffler, F. E. (2007). The Dehalococcoides Population in Sediment-Free Mixed Cultures Metabolically Dechlorinates the Commercial Polychlorinated Biphenyl Mixture Aroclor 1260. Appl. Environ. Microbiol. 73: 2513-2521 [Abstract] [Full Text]  
  • Morris, R. M., Fung, J. M., Rahm, B. G., Zhang, S., Freedman, D. L., Zinder, S. H., Richardson, R. E. (2007). Comparative Proteomics of Dehalococcoides spp. Reveals Strain-Specific Peptides Associated with Activity. Appl. Environ. Microbiol. 73: 320-326 [Abstract] [Full Text]  
  • Lee, P. K. H., Johnson, D. R., Holmes, V. F., He, J., Alvarez-Cohen, L. (2006). Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of Dehalococcoides spp.. Appl. Environ. Microbiol. 72: 6161-6168 [Abstract] [Full Text]  
  • Bedard, D. L., Bailey, J. J., Reiss, B. L., Jerzak, G. V. S. (2006). Development and Characterization of Stable Sediment-Free Anaerobic Bacterial Enrichment Cultures That Dechlorinate Aroclor 1260. Appl. Environ. Microbiol. 72: 2460-2470 [Abstract] [Full Text]  
  • Ritalahti, K. M., Amos, B. K., Sung, Y., Wu, Q., Koenigsberg, S. S., Loffler, F. E. (2006). Quantitative PCR Targeting 16S rRNA and Reductive Dehalogenase Genes Simultaneously Monitors Multiple Dehalococcoides Strains. Appl. Environ. Microbiol. 72: 2765-2774 [Abstract] [Full Text]  
  • Sung, Y., Ritalahti, K. M., Apkarian, R. P., Loffler, F. E. (2006). Quantitative PCR Confirms Purity of Strain GT, a Novel Trichloroethene-to-Ethene-Respiring Dehalococcoides Isolate.. Appl. Environ. Microbiol. 72: 1980-1987 [Abstract] [Full Text]  
  • Grostern, A., Edwards, E. A. (2006). Growth of Dehalobacter and Dehalococcoides spp. during Degradation of Chlorinated Ethanes. Appl. Environ. Microbiol. 72: 428-436 [Abstract] [Full Text]  
  • Fagervold, S. K., Watts, J. E. M., May, H. D., Sowers, K. R. (2005). Sequential Reductive Dechlorination of meta-Chlorinated Polychlorinated Biphenyl Congeners in Sediment Microcosms by Two Different Chloroflexi Phylotypes. Appl. Environ. Microbiol. 71: 8085-8090 [Abstract] [Full Text]  
  • Yoshida, N., Takahashi, N., Hiraishi, A. (2005). Phylogenetic Characterization of a Polychlorinated-Dioxin- Dechlorinating Microbial Community by Use of Microcosm Studies. Appl. Environ. Microbiol. 71: 4325-4334 [Abstract] [Full Text]  
  • Watts, J. E. M., Fagervold, S. K., May, H. D., Sowers, K. R. (2005). A PCR-based specific assay reveals a population of bacteria within the Chloroflexi associated with the reductive dehalogenation of polychlorinated biphenyls. Microbiology 151: 2039-2046 [Abstract] [Full Text]  
  • Seshadri, R., Adrian, L., Fouts, D. E., Eisen, J. A., Phillippy, A. M., Methe, B. A., Ward, N. L., Nelson, W. C., Deboy, R. T., Khouri, H. M., Kolonay, J. F., Dodson, R. J., Daugherty, S. C., Brinkac, L. M., Sullivan, S. A., Madupu, R., Nelson, K. E., Kang, K. H., Impraim, M., Tran, K., Robinson, J. M., Forberger, H. A., Fraser, C. M., Zinder, S. H., Heidelberg, J. F. (2005). Genome Sequence of the PCE-Dechlorinating Bacterium Dehalococcoides ethenogenes. Science 307: 105-108 [Abstract] [Full Text]  
  • Macbeth, T. W., Cummings, D. E., Spring, S., Petzke, L. M., Sorenson, K. S. Jr. (2004). Molecular Characterization of a Dechlorinating Community Resulting from In Situ Biostimulation in a Trichloroethene-Contaminated Deep, Fractured Basalt Aquifer and Comparison to a Derivative Laboratory Culture. Appl. Environ. Microbiol. 70: 7329-7341 [Abstract] [Full Text]  
  • Krajmalnik-Brown, R., Holscher, T., Thomson, I. N., Saunders, F. M., Ritalahti, K. M., Loffler, F. E. (2004). Genetic Identification of a Putative Vinyl Chloride Reductase in Dehalococcoides sp. Strain BAV1. Appl. Environ. Microbiol. 70: 6347-6351 [Abstract] [Full Text]  
  • Holscher, T., Krajmalnik-Brown, R., Ritalahti, K. M., von Wintzingerode, F., Gorisch, H., Loffler, F. E., Adrian, L. (2004). Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides. Appl. Environ. Microbiol. 70: 5290-5297 [Abstract] [Full Text]  
  • Duhamel, M., Mo, K., Edwards, E. A. (2004). Characterization of a Highly Enriched Dehalococcoides-Containing Culture That Grows on Vinyl Chloride and Trichloroethene. Appl. Environ. Microbiol. 70: 5538-5545 [Abstract] [Full Text]  
  • Muller, J. A., Rosner, B. M., von Abendroth, G., Meshulam-Simon, G., McCarty, P. L., Spormann, A. M. (2004). Molecular Identification of the Catabolic Vinyl Chloride Reductase from Dehalococcoides sp. Strain VS and Its Environmental Distribution. Appl. Environ. Microbiol. 70: 4880-4888 [Abstract] [Full Text]  
  • Ritalahti, K. M., Loffler, F. E. (2004). Populations Implicated in Anaerobic Reductive Dechlorination of 1,2-Dichloropropane in Highly Enriched Bacterial Communities. Appl. Environ. Microbiol. 70: 4088-4095 [Abstract] [Full Text]  
  • Empadinhas, N., Albuquerque, L., Costa, J., Zinder, S. H., Santos, M. A. S., Santos, H., da Costa, M. S. (2004). A Gene from the Mesophilic Bacterium Dehalococcoides ethenogenes Encodes a Novel Mannosylglycerate Synthase. J. Bacteriol. 186: 4075-4084 [Abstract] [Full Text]  
  • Yang, Y., Zeyer, J. (2003). Specific Detection of Dehalococcoides Species by Fluorescence In Situ Hybridization with 16S rRNA-Targeted Oligonucleotide Probes. Appl. Environ. Microbiol. 69: 2879-2883 [Abstract] [Full Text]  
  • Cupples, A. M., Spormann, A. M., McCarty, P. L. (2003). Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR. Appl. Environ. Microbiol. 69: 953-959 [Abstract] [Full Text]  
  • He, J., Ritalahti, K. M., Aiello, M. R., Loffler, F. E. (2003). Complete Detoxification of Vinyl Chloride by an Anaerobic Enrichment Culture and Identification of the Reductively Dechlorinating Population as a Dehalococcoides Species. Appl. Environ. Microbiol. 69: 996-1003 [Abstract] [Full Text]  
  • Coleman, N. V., Mattes, T. E., Gossett, J. M., Spain, J. C. (2002). Phylogenetic and Kinetic Diversity of Aerobic Vinyl Chloride-Assimilating Bacteria from Contaminated Sites. Appl. Environ. Microbiol. 68: 6162-6171 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»