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Applied and Environmental Microbiology, February 2002, p. 582-587, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.582-587.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

L-Glucitol Catabolism in Stenotrophomonas maltophilia Ac

Elke Brechtel, Alexander Huwig, and Friedrich Giffhorn*

Lehrstuhl für Angewandte Mikrobiologie, Universität des Saarlandes, D-66041 Saarbrücken, Germany

Received 18 June 2001/ Accepted 6 November 2001

The carbohydrate catabolism of the bacterium Stenotrophomonas maltophilia Ac (previously named Pseudomonas sp. strain Ac), which is known to convert the unnatural polyol L-glucitol to D-sorbose during growth on the former as the sole source of carbon and energy, was studied in detail. All enzymes operating in a pathway that channels L-glucitol via D-sorbose into compounds of the intermediary metabolism were demonstrated, and for some prominent reactions the products of conversion were identified. D-Sorbose was converted by C-3 epimerization to D-tagatose, which, in turn, was isomerized to D-galactose. D-Galactose was the initial substrate of the De Ley-Doudoroff pathway, involving reactions of NAD-dependent oxidation of D-galactose to D-galactonate, its dehydration to 2-keto-3-deoxy-D-galactonate, and its phosphorylation to 2-keto-3-deoxy-D-galactonate 6-phosphate. Finally, aldol cleavage yielded pyruvate and D-glycerate 3-phosphate as the central metabolic intermediates.

* Corresponding author. Mailing address: Lehrstuhl für Angewandte Mikrobiologie, Universität des Saarlandes, Postfach 15 11 50, D-66041 Saarbrücken, Germany. Phone: 49-(0)681-302-2704. Fax: 49-(0)681-302-4360. E-mail: giffhorn{at}mx.uni-saarland.de.

Applied and Environmental Microbiology, February 2002, p. 582-587, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.582-587.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Copyright © 2002 by the American Society for Microbiology. All rights reserved.