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Applied and Environmental Microbiology, February 2002, p. 597-601, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.597-601.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Fungitoxicity Assays for Inhibition of Germination-Associated Adhesion of Botrytis cinerea and Puccinia recondita Spores

Richard A. Slawecki, Eileen P. Ryan, and David H. Young*

Rohm and Haas Company, Spring House Technical Center, Spring House, Pennsylvania 19477

Received 16 July 2001/ Accepted 29 November 2001

Botrytis cinerea and Puccinia recondita spores adhere strongly to polystyrene microtiter plates coincident with germination. We developed assays for inhibition of spore adhesion in 96-well microtiter plates by using sulforhodamine B staining to quantify the adherent spores. In both organisms, fungicides that inhibited germination strongly inhibited spore adhesion, with 50% effective concentrations (EC50s) comparable to those for inhibition of germination. In contrast, fungicides that acted after germination in B. cinerea inhibited spore adhesion to microtiter plates only at concentrations much higher than their EC50s for inhibition of mycelial growth. Similarly, in P. recondita the ergosterol biosynthesis inhibitors myclobutanil and fenbuconazole acted after germination and did not inhibit spore adhesion. The assays provide a rapid, high-throughput alternative to traditional spore germination assays and may be applicable to other fungi.

* Corresponding author. Mailing address: Dow AgroSciences, 9330 Zionsville Rd., Indianapolis, IN 46268. Phone: (317) 337-3322. Fax: (317) 337-3249. E-mail: dyoung{at}dow.com.

Applied and Environmental Microbiology, February 2002, p. 597-601, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.597-601.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cotoras, M., Silva, E. (2005). Differences in the initial events of infection of Botrytis cinerea strains isolated from tomato and grape. Mycologia 97: 485-492 [Abstract] [Full Text]  


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