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Applied and Environmental Microbiology, February 2002, p. 616-622, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.616-622.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of ActA, Ami, InlB, and Listeriolysin O in Listeria monocytogenes of Human and Food Origin

C. Jacquet,1* E. Gouin,2 D. Jeannel,3 P. Cossart,2 and J. Rocourt1

Laboratoire des Listeria, Centre National de Référence des Listeria-World Health Organization Collaborating Center for Foodborne Listeriosis,1 Unité des Interactions Bactéries-Cellules,,2 Centre de Recherche Clinique, Institut Pasteur, 75724 Paris Cedex 15, France3

Received 12 February 2001/ Accepted 1 November 2001

Expression of proteins involved in the adhesion of Listeria monocytogenes to mammalian cells or in the intracellular life cycle of this bacterium, including listeriolysin O (LLO), ActA, Ami, and InlB, was used to compare two populations of L. monocytogenes strains. One of the populations comprised 300 clinical strains, and the other comprised 150 food strains. All strains expressed LLO, InlB, and ActA. No polymorphism was observed for LLO and InlB. Ami was detected in 283 of 300 human strains and in 149 of 150 food strains. The strains in which Ami was not detected were serovar 4b strains. Based on the molecular weights of the proteins detected, the strains were divided into two groups with Ami (groups Ami1 [75% of the strains] and Ami2 [21%]) and into four groups with ActA (groups ActA1 [52% of the strains], ActA2 [18%], ActA3 [30%], and ActA4 [one strain isolated from food]). Logistic regression showed that food strains were more likely to belong to group ActA3 than human strains (odds ratio [OR] = 2.90; P = 1 x 10-4). Of the strains isolated from patients with non-pregnancy-related cases of listeriosis, bacteremia was predominantly associated with group Ami1 strains (OR = 1.89; P = 1 x 10-2) and central nervous system infections were associated with group ActA2 strains (OR = 3.04; P = 1 x 10-3) and group ActA3 strains (OR = 3.91; P = 1 x 10-3).

* Corresponding author. Mailing address: Laboratoire des Listeria, Centre National de Reférénce des Listeria-World Health Organization Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25-28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 40 61 31 12. Fax: 33 1 40 61 35 67. E-mail: christine.jacquet{at}pasteur.fr.

Applied and Environmental Microbiology, February 2002, p. 616-622, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.616-622.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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