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Applied and Environmental Microbiology, February 2002, p. 642-649, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.642-649.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Tetrachloroethene Dehalorespiration and Growth of Desulfitobacterium frappieri TCE1 in Strict Dependence on the Activity of Desulfovibrio fructosivorans

Oliver Drzyzga1* and Jan C. Gottschal2

Center for Environmental Research and Environmental Technology (UFT), Department of Marine Microbiology, University of Bremen, 28359 Bremen, Germany,1 Microbial Ecology Section, Department of Microbiology, University of Groningen, 9751 NN Haren, The Netherlands2

Received 16 July 2001/ Accepted 6 November 2001

Tetrachloroethene (PCE) dehalorespiration was investigated in a continuous coculture of the sulfate-reducing bacterium Desulfovibrio fructosivorans and the dehalorespiring Desulfitobacterium frappieri TCE1 at different sulfate concentrations and in the absence of sulfate. Fructose (2.5 mM) was the single electron donor, which could be used only by the sulfate reducer. With 2.5 mM sulfate, the dehalogenating strain was outnumbered by the sulfate-reducing bacterium, sulfate reduction was the dominating process, and only trace amounts of PCE were dehalogenated by strain TCE1. With 1 mM sulfate in the medium, complete sulfate reduction and complete PCE dehalogenation to cis-dichloroethene (cis-DCE) occurred. In the absence of sulfate, PCE was also completely dehalogenated to cis-DCE, and the population size of strain TCE1 increased significantly. The results presented here describe for the first time dehalogenation of PCE by a dehalorespiring anaerobe in strict dependence on the activity of a sulfate-reducing bacterium with a substrate that is exclusively used by the sulfate reducer. This interaction was studied under strictly controlled and quantifiable conditions in continuous culture and shown to depend on interspecies hydrogen transfer under sulfate-depleted conditions. Interspecies hydrogen transfer was demonstrated by direct H2 measurements of the gas phase and by the production of methane after the addition of a third organism, Methanobacterium formicicum.

* Corresponding author. Mailing address: Universität Bremen, Zentrum für Umweltforschung und Umwelttechnologie (UFT), Abteilung Marine Mikrobiologie, Leobener Strasse, 28359 Bremen, Germany. Phone: 49 421-218-7234. Fax: 49 421-218-7222. E-mail: drzyzga{at}biotec.uni-bremen.de.

Applied and Environmental Microbiology, February 2002, p. 642-649, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.642-649.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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