This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chavant, P.
Right arrow Articles by Hebraud, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chavant, P.
Right arrow Articles by Hebraud, M.
Agricola
Right arrow Articles by Chavant, P.
Right arrow Articles by Hebraud, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2002, p. 728-737, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.728-737.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Listeria monocytogenes LO28: Surface Physicochemical Properties and Ability To Form Biofilms at Different Temperatures and Growth Phases

Patrick Chavant,1 Brigitte Martinie,2 Thierry Meylheuc,3 Marie-Noëlle Bellon-Fontaine,3 and Michel Hebraud1*

Station de Recherches sur la Viande-Microbiologie,1 Laboratoire de Microbiologie, Institut National de la Recherche Agronomique, Saint-Genès Champanelle,2 Unité de Recherche en Bioadhésion et Hygiène des Matériaux, Institut National de la Recherche Agronomique, Massy, France3

Received 1 May 2001/ Accepted 31 October 2001

The surface physicochemical properties of Listeria monocytogenes LO28 under different conditions (temperature and growth phase) were determined by use of microelectrophoresis and microbial adhesion to solvents. The effect of these parameters on adhesion and biofilm formation by L. monocytogenes LO28 on hydrophilic (stainless steel) and hydrophobic (polytetrafluoroethylene [PTFE]) surfaces was assessed. The bacterial cells were always negatively charged and possessed hydrophilic surface properties, which were negatively correlated with growth temperature. The colonization of the two surfaces, monitored by scanning electron microscopy, epifluorescence microscopy, and cell enumeration, showed that the strain had a great capacity to colonize both surfaces whatever the incubation temperature. However, biofilm formation was faster on the hydrophilic substratum. After 5 days at 37 or 20°C, the biofilm structure was composed of aggregates with a three-dimensional shape, but significant detachment took place on PTFE at 37°C. At 8°C, only a bacterial monolayer was visible on stainless steel, while no growth was observed on PTFE. The growth phase of bacteria used to inoculate surfaces had a significant effect only in some cases during the first steps of biofilm formation. The surface physicochemical properties of the strain are correlated with adhesion and surface colonization.

* Corresponding author. Mailing address: SRV-Microbiologie, INRA de Theix, 63122 Saint-Genès Champanelle, France. Phone: 33 4 73 62 46 70. Fax: 33 4 73 62 42 68. E-mail: hebraud{at}clermont.inra.fr.

Applied and Environmental Microbiology, February 2002, p. 728-737, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.728-737.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poimenidou, S., Belessi, C. A., Giaouris, E. D., Gounadaki, A. S., Nychas, G.-J. E., Skandamis, P. N. (2009). Listeria monocytogenes Attachment to and Detachment from Stainless Steel Surfaces in a Simulated Dairy Processing Environment. Appl. Environ. Microbiol. 75: 7182-7188 [Abstract] [Full Text]  
  • Rieu, A., Briandet, R., Habimana, O., Garmyn, D., Guzzo, J., Piveteau, P. (2008). Listeria monocytogenes EGD-e Biofilms: No Mushrooms but a Network of Knitted Chains. Appl. Environ. Microbiol. 74: 4491-4497 [Abstract] [Full Text]  
  • Gyo, M., Nikaido, T., Okada, K., Yamauchi, J., Tagami, J., Matin, K. (2008). Surface Response of Fluorine Polymer-Incorporated Resin Composites to Cariogenic Biofilm Adherence. Appl. Environ. Microbiol. 74: 1428-1435 [Abstract] [Full Text]  
  • Gamble, R., Muriana, P. M. (2007). Microplate Fluorescence Assay for Measurement of the Ability of Strains of Listeria monocytogenes from Meat and Meat-Processing Plants To Adhere to Abiotic Surfaces. Appl. Environ. Microbiol. 73: 5235-5244 [Abstract] [Full Text]  
  • Pan, Y., Breidt, F. Jr., Kathariou, S. (2006). Resistance of Listeria monocytogenes Biofilms to Sanitizing Agents in a Simulated Food Processing Environment. Appl. Environ. Microbiol. 72: 7711-7717 [Abstract] [Full Text]  
  • Challan Belval, S., Gal, L., Margiewes, S., Garmyn, D., Piveteau, P., Guzzo, J. (2006). Assessment of the Roles of LuxS, S-Ribosyl Homocysteine, and Autoinducer 2 in Cell Attachment during Biofilm Formation by Listeria monocytogenes EGD-e. Appl. Environ. Microbiol. 72: 2644-2650 [Abstract] [Full Text]  
  • Walker, S. L., Hill, J. E., Redman, J. A., Elimelech, M. (2005). Influence of Growth Phase on Adhesion Kinetics of Escherichia coli D21g. Appl. Environ. Microbiol. 71: 3093-3099 [Abstract] [Full Text]  
  • Schirm, M., Kalmokoff, M., Aubry, A., Thibault, P., Sandoz, M., Logan, S. M. (2004). Flagellin from Listeria monocytogenes Is Glycosylated with {beta}-O-Linked N-Acetylglucosamine. J. Bacteriol. 186: 6721-6727 [Abstract] [Full Text]  
  • Yang, H.-H., Vinopal, R. T., Grasso, D., Smets, B. F. (2004). High Diversity among Environmental Escherichia coli Isolates from a Bovine Feedlot. Appl. Environ. Microbiol. 70: 1528-1536 [Abstract] [Full Text]  
  • Djordjevic, D., Wiedmann, M., McLandsborough, L. A. (2002). Microtiter Plate Assay for Assessment of Listeria monocytogenes Biofilm Formation. Appl. Environ. Microbiol. 68: 2950-2958 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»