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Applied and Environmental Microbiology, February 2002, p. 917-922, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.917-922.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis

L. G. Bermúdez-Humarán,1,2 P. Langella,1* A. Miyoshi,1,3 A. Gruss,1 R. Tamez Guerra,2 R. Montes de Oca-Luna,2 and Y. Le Loir1

Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France,1 Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolas de los Garza, Nuevo León, Mexico,2 Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, Brazil3

Received 19 July 2001/ Accepted 15 October 2001

The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer.

* Corresponding author. Mailing address: Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France. Phone: 33 01 34 65 20 83. Fax: 33 01 34 65 20 65. E-mail: langella{at}biotec.jouy.inra.fr.

Applied and Environmental Microbiology, February 2002, p. 917-922, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.917-922.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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