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Applied and Environmental Microbiology, February 2002, p. 938-941, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.938-941.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Streptococcus thermophilus Is Able To Produce a ß-Galactosidase Active during Its Transit in the Digestive Tract of Germ-Free Mice

Sophie Drouault,1 Jamila Anba,2 and Gérard Corthier2*

Arilait Recherches, 75009 Paris,1 Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France2

Received 25 May 2001/ Accepted 30 October 2001

This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active ß-galactosidase in the digestive tract, although it did not multiply during its transit. This production was enhanced when lactose (the inducer) was added to the diet.

* Corresponding author. Mailing address: Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France. Phone: 33-1 34 65 24 67. Fax: 33-1 34 65 24 62. E-mail: corthier{at}jouy.inra.fr.

Applied and Environmental Microbiology, February 2002, p. 938-941, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.938-941.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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