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Applied and Environmental Microbiology, February 2002, p. 952-956, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.952-956.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Two-Step Procedure for Large-Scale Purification of Pediocin-Like Bacteriocins and Other Cationic Antimicrobial Peptides from Complex Culture Medium

Marianne Uteng, Håvard Hildeng Hauge, Ilia Brondz, Jon Nissen-Meyer, and Gunnar Fimland*

Department of Biochemistry, University of Oslo, Oslo, Norway

Received 17 August 2001/ Accepted 26 November 2001

A rapid and simple two-step procedure suitable for both small- and large-scale purification of pediocin-like bacteriocins and other cationic peptides has been developed. In the first step, the bacterial culture was applied directly on a cation-exchange column (1-ml cation exchanger per 100-ml cell culture). Bacteria and anionic compounds passed through the column, and cationic bacteriocins were subsequently eluted with 1 M NaCl. In the second step, the bacteriocin fraction was applied on a low-pressure, reverse-phase column and the bacteriocins were detected as major optical density peaks upon elution with propanol. More than 80% of the activity that was initially in the culture supernatant was recovered in both purification steps, and the final bacteriocin preparation was more than 90% pure as judged by analytical reverse-phase chromatography and capillary electrophoresis.

* Corresponding author. Mailing address: Department of Biochemistry, University of Oslo, Post Box 1041, Blindern, 0316 Oslo, Norway. Phone: 47-22 85 66 32. Fax: 47-22 85 44 43. E-mail: gunnar.fimland{at}biokjemi.uio.no.

Applied and Environmental Microbiology, February 2002, p. 952-956, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.952-956.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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