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Applied and Environmental Microbiology, February 2002, p. 957-962, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.957-962.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Coexistence of Two Distinct Copies of Naphthalene Degradation Genes in Pseudomonas Strains Isolated from the Western Mediterranean Region

Marcela Ferrero,,{dagger} Enrique Llobet-Brossa, Jorge Lalucat,,{ddagger} Elena García-Valdés, Ramón Rosselló-Mora, and Rafael Bosch*

Departament de Biologia, Microbiologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07071 Palma de Mallorca, Spain

Received 28 June 2001/ Accepted 16 November 2001

We analyzed the occurrence of the naphthalene degradation upper-pathway (nah) genes in the western Mediterranean region. The amplification, restriction, and sequence analysis of internal fragments for several nah genes (nahAc, nahB, nahC, and nahE) from naphthalene-degrading strains isolated from this geographical area proved the coexistence of two distinct sets of nah genes.

* Corresponding author. Mailing address: Departament de Biologia, Microbiologia, Universitat de les Illes Balears, Carretera Valldemossa, km 7.5, E-07071, Palma de Mallorca, Spain. Phone: 34-971-173141. Fax: 34-971-173184. E-mail: rbosch{at}uib.es.

{dagger} Present address: PROIMI-Biotecnología, CP 4000, San Miguel de Tucumán, Argentina.

{ddagger} Present address: Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany.

Applied and Environmental Microbiology, February 2002, p. 957-962, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.957-962.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.