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Applied and Environmental Microbiology, February 2002, p. 977-980, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.977-980.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Probiotic Lactobacillus Biosurfactant Proteins by ProteinChip Tandem Mass Spectrometry Tryptic Peptide Sequencing

Gregor Reid,1,2,3 Bing Siang Gan,1,2,4,5,6 Yi-Min She,7 Werner Ens,7 Scot Weinberger,8 and Jeffrey C. Howard1,2,3,6,9*

Lawson Health Research Institute,1 Hand and Upper Limb Centre, Cell and Molecular Biology Laboratory, St. Joseph's Health Care,6 Departments of Surgery,2 Microbiology and Immunology,3 Biochemistry,9 Pharmacology and Toxicology,4 Medical Biophysics, University of Western Ontario, London, Ontario,5 Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada,7 Ciphergen Biosystems, Inc., Fremont, California8

Received 26 July 2001/ Accepted 9 November 2001

A novel ProteinChip-interfaced tandem mass spectrometer was employed to identify collagen binding proteins from biosurfactant produced by Lactobacillus fermentum RC-14. On-chip tryptic digestion of the captured collagen binding proteins resulted in rapid sequence identification of five novel tryptic peptide sequences via collision-induced dissociation tandem mass spectrometry.

* Corresponding author. Mailing address: Lawson Health Research Institute, St. Joseph's Health Care, Room G518, 268 Grosvenor St., London, Ontario N6A 4V2, Canada. Phone: (519) 646-6000, ext. 64167. Fax: (519) 646-6049. E-mail: jhoward{at}lri.sjhc.london.on.ca.

Applied and Environmental Microbiology, February 2002, p. 977-980, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.977-980.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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