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Applied and Environmental Microbiology, February 2002, p. 995-998, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.995-998.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Simple Procedure for Rapid Identification of Vibrio cholerae from the Aquatic Environment

Nipa Choopun,1 Valérie Louis,1 Anwar Huq,1,2 and Rita R. Colwell1,2*

Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202 ,1 Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 207422

Received 6 August 2001/ Accepted 2 November 2001

Biochemical tests commonly used to screen for Vibrio cholerae in environmental samples were evaluated, and we found that a combination of alkaline peptone enrichment followed by streaking on thiosulfate citrate bile salts sucrose agar and testing for arginine dihydrolase activity and esculin hydrolysis was an effective rapid technique to screen for aquatic environmental V. cholerae. This technique provided 100% sensitivity and >=70% specificity.

* Corresponding author. Mailing address: Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt Street, Baltimore, MD 21202. Phone: (703) 292-8000. Fax: (703) 292-9232. E-mail: colwell{at}umbi.umd.edu.

Applied and Environmental Microbiology, February 2002, p. 995-998, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.995-998.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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