This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishige, T.
Right arrow Articles by Kato, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishige, T.
Right arrow Articles by Kato, N.
Agricola
Right arrow Articles by Ishige, T.
Right arrow Articles by Kato, N.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2002, p. 1192-1195, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1192-1195.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Wax Ester Production from n-Alkanes by Acinetobacter sp. Strain M-1: Ultrastructure of Cellular Inclusions and Role of Acyl Coenzyme A Reductase

Takeru Ishige,1 Akio Tani,1 Keiji Takabe,2 Kazunori Kawasaki,3 Yasuyoshi Sakai,1 and Nobuo Kato1*

Divisions of Applied Life Sciences,1 Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502,2 National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central, Tsukuba 305-8561, Japan3

Received 4 September 2001/ Accepted 20 November 2001

Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.

* Corresponding author. Mailing address: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. Phone and fax: 81-75-753-6385. E-mail: nkato{at}kais.kyoto-u.ac.jp.

Applied and Environmental Microbiology, March 2002, p. 1192-1195, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1192-1195.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gaffoor, I., Brown, D. W., Plattner, R., Proctor, R. H., Qi, W., Trail, F. (2005). Functional Analysis of the Polyketide Synthase Genes in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum). Eukaryot Cell 4: 1926-1933 [Abstract] [Full Text]  
  • Waltermann, M., Steinbuchel, A. (2005). Neutral Lipid Bodies in Prokaryotes: Recent Insights into Structure, Formation, and Relationship to Eukaryotic Lipid Depots. J. Bacteriol. 187: 3607-3619 [Full Text]  
  • Van Hamme, J. D., Singh, A., Ward, O. P. (2003). Recent Advances in Petroleum Microbiology. Microbiol. Mol. Biol. Rev. 67: 503-549 [Abstract] [Full Text]  
  • Bogan, B. W., Sullivan, W. R., Kayser, K. J., Derr, K., Aldrich, H. C., Paterek, J. R. (2003). Alkanindiges illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield soils. Int. J. Syst. Evol. Microbiol. 53: 1389-1395 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»