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Applied and Environmental Microbiology, March 2002, p. 1240-1249, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1240-1249.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Nitrous Oxide Formation in the Colne Estuary, England: the Central Role of Nitrite

Liang F. Dong, David B. Nedwell,^* Graham J. C. Underwood, Daniel C. O. Thornton,^, and Iman Rusmana

Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom

Received 17 July 2001/ Accepted 4 December 2001

Nitrate and nitrite concentrations in the water and nitrous oxide and nitrite fluxes across the sediment-water interface were measured monthly in the River Colne estuary, England, from December 1996 to March 1998. Water column concentrations of N₂O in the Colne were supersaturated with respect to air, indicating that the estuary was a source of N₂O for the atmosphere. At the freshwater end of the estuary, nitrous oxide effluxes from the sediment were closely correlated with the nitrite concentrations in the overlying water and with the nitrite influx into the sediment. Increases in N₂O production from sediments were about 10 times greater with the addition of nitrite than with the addition of nitrate. Rates of denitrification were stimulated to a larger extent by enhanced nitrite than by nitrate concentrations. At 550 µM nitrite or nitrate (the highest concentration used), the rates of denitrification were 600 µmol N · m^-2 · h^-1 with nitrite but only 180 µmol N · m^-2 · h^-1 with nitrate. The ratios of rates of nitrous oxide production and denitrification (N₂O/N₂ x 100) were significantly higher with the addition of nitrite (7 to 13% of denitrification) than with nitrate (2 to 4% of denitrification). The results suggested that in addition to anaerobic bacteria, which possess the complete denitrification pathway for N₂ formation in the estuarine sediments, there may be two other groups of bacteria: nitrite denitrifiers, which reduce nitrite to N₂ via N₂O, and obligate nitrite-denitrifying bacteria, which reduce nitrite to N₂O as the end product. Consideration of free-energy changes during N₂O formation led to the conclusion that N₂O formation using nitrite as the electron acceptor is favored in the Colne estuary and may be a critical factor regulating the formation of N₂O in high-nutrient-load estuaries.

Present address: Ecology Centre, University of Sunderland, Sunderland SR1 3SD, United Kingdom.

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