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Applied and Environmental Microbiology, March 2002, p. 1381-1391, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1381-1391.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity

Marc Vancanneyt,1* Angiolella Lombardi,2 Christian Andrighetto,2 Edo Knijff,3 Sandra Torriani,3,4 K. Johanna Björkroth,5 Charles M. A. P. Franz,6 María R. Foulquié Moreno,7 Hilde Revets,8 Luc De Vuyst,7 Jean Swings,1 Karel Kersters,1 Franco Dellaglio,3 and Wilhelm H. Holzapfel6

BCCM/LMG Bacteria Collection, Laboratory of Microbiology, University of Ghent, B-9000 Ghent,1 Research Group of Industrial Microbiology, Fermentation Technology, and Downstream Processing, Vrije Universiteit Brussel, B-1050 Brussels,7 Department of Immunology, Parasitology, and Ultrastructure, Flemish Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, B-1640 Sint-Genesius-Rode, Belgium,8 Veneto Agricoltura-Istituto per la Qualità e le Tecnologie Agroalimentari, I-36016 Thiene,2 Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, I-37134 Verona ,3 DOFATA, University of Catania, I-95124 Catania, Italy,4 Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, SF-00014 Helsinki, Finland,5 Institute for Biotechnology and Molecular Biology, Federal Research Center for Nutrition, D-76131 Karlsruhe, Germany6

Received 9 July 2001/ Accepted 12 December 2001

Seventy-eight Enterococcus faecium strains from various sources were characterized by random amplified polymorphic DNA (RAPD)-PCR, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE) analysis of SmaI restriction patterns. Two main genomic groups (I and II) were obtained in both RAPD-PCR and AFLP analyses. DNA-DNA hybridization values between representative strains of both groups demonstrated a mean DNA-DNA reassociation level of 71%. PFGE analysis revealed high genetic strain diversity within the two genomic groups. Only group I contained strains originating from human clinical samples or strains that were vancomycin-resistant or beta-hemolytic. No differentiating phenotypic features between groups I and II were found using the rapid ID 32 STREP system. The two groups could be further subdivided into, respectively, four and three subclusters in both RAPD-PCR and AFLP analyses, and a high correlation was seen between the subclusters generated by these two methods. Subclusters of group I were to some extent correlated with origin, pathogenicity, and bacteriocinogeny of the strains. Host specificity of E. faecium strains was not confirmed.

* Corresponding author. Mailing address: BCCM/LMG Bacteria Collection, Laboratory of Microbiology, University of Ghent, Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: 32 9 2645115. Fax: 32 9 2645092. E-mail: Marc.Vancanneyt{at}rug.ac.be.

Applied and Environmental Microbiology, March 2002, p. 1381-1391, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1381-1391.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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