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Applied and Environmental Microbiology, March 2002, p. 1458-1463, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1458-1463.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rupture of the Cell Envelope by Decompression of the Deep-Sea Methanogen Methanococcus jannaschii

Chan Beum Park and Douglas S. Clark*

Department of Chemical Engineering, University of California, Berkeley, California 94720

Received 15 August 2001/ Accepted 4 December 2001

The effect of decompression on the structure of Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen, was studied in a novel high-pressure, high-temperature bioreactor. The cell envelope of M. jannaschii appeared to rupture upon rapid decompression (ca. 1 s) from 260 atm of hyperbaric pressure. When decompression from 260 atm was performed over 5 min, the proportion of ruptured cells decreased significantly. In contrast to the effect produced by decompression from hyperbaric pressure, decompression from a hydrostatic pressure of 260 atm did not induce cell lysis.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of California, Berkeley, CA 94720-1462. Phone: (510) 642-2408. Fax: (510) 643-1228. E-mail: clark{at}cchem.berkeley.edu.

Applied and Environmental Microbiology, March 2002, p. 1458-1463, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1458-1463.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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