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Applied and Environmental Microbiology, March 2002, p. 1464-1467, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1464-1467.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Continuous Steady-State Method Using Tenax for Delivering Tetrachloroethene to Chloro-Respiring Bacteria

Rachel A. Brennan and Robert A. Sanford*

Department of Civil & Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

Received 24 July 2001/ Accepted 14 December 2001

Tenax-TA, a solid-phase sorbent, was used as an alternative to hexadecane for continuous delivery of tetrachloroethene (PCE) to Desulfuromonas strain BB1, a chloro-respiring microorganism. In both batch and bioreactor configurations, Tenax not only maintained low, steady-state concentrations of PCE in an active culture for several months but also adsorbed the product of dechlorination, cis-1,2-dichloroethene, before it approached toxic levels.

* Corresponding author. Mailing address: Department of Civil & Environmental Engineering, University of Illinois at Urbana-Champaign, 3230C Newmark Civil Engineering Laboratory, 205 N. Mathews Ave., Urbana, IL 61801. Phone: (217) 244-7250. Fax: (217) 333-6968. E-mail: rsanford{at}uiuc.edu.

Applied and Environmental Microbiology, March 2002, p. 1464-1467, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1464-1467.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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