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Applied and Environmental Microbiology, April 2002, p. 1556-1560, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1556-1560.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

EglC, a New Endoglucanase from Aspergillus niger with Major Activity towards Xyloglucan

Alinda A. Hasper, Ester Dekkers,,{dagger} Marc van Mil, Peter J. I. van de Vondervoort,,{dagger} and Leo H. de Graaff*

Molecular Genetics of Industrial Microorganisms Section, Wageningen University, NL-6703 HA Wageningen, The Netherlands

Received 17 August 2001/ Accepted 11 January 2002

A novel gene, eglC, encoding an endoglucanase, was cloned from Aspergillus niger. Transcription of eglC is regulated by XlnR, a transcriptional activator that controls the degradation of polysaccharides in plant cell walls. EglC is an 858-amino-acid protein and contains a conserved C-terminal cellulose-binding domain. EglC can be classified in glycoside hydrolase family 74. No homology to any of the endoglucanases from Trichoderma reesei was found. In the plant cell wall xyloglucan is closely linked to cellulose fibrils. We hypothesize that the EglC cellulose-binding domain anchors the enzyme to the cellulose chains while it is cleaving the xyloglucan backbone. By this action it may contribute to the degradation of the plant cell wall structure together with other enzymes, including hemicellulases and cellulases. EglC is most active towards xyloglucan and therefore is functionally different from the other two endoglucanases from A. niger, EglA and EglB, which exhibit the greatest activity towards ß-glucan. Although the mode of action of EglC is not known, this enzyme represents a new enzyme function involved in plant cell wall polysaccharide degradation by A. niger.

* Corresponding author. Mailing address: Section Molecular Genetics of Industrial Microorganisms, Wageningen University, Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands. Phone: 31 317 484691. Fax: 31 317 484011. E-mail: leo.degraaff{at}algemeen.mgim.wau.nl.

{dagger} Present address: Laboratory of Phytopathology, Wageningen University, NL-6709 PD Wageningen, The Netherlands.

Applied and Environmental Microbiology, April 2002, p. 1556-1560, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1556-1560.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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