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Applied and Environmental Microbiology, April 2002, p. 1652-1657, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1652-1657.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effects of Temperature, Water Activity, and Syrup Film Composition on the Growth of Wallemia sebi: Development and Assessment of a Model Predicting Growth Lags in Syrup Agar and Crystalline Sugar

Jannik Vindeløv and Nils Arneborg*

Department of Dairy and Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg C, Denmark

Received 28 June 2001/ Accepted 14 January 2002

We investigated the effects of temperature, water activity (aw), and syrup film composition on the CFU growth of Wallemia sebi in crystalline sugar. At a high aw (0.82) at both high (20°C) and low (10°C) temperatures, the CFU growth of W. sebi in both white and extrawhite sugar could be described using a modified Gompertz model. At a low aw (0.76), however, the modified Gompertz model could not be fitted to the CFU data obtained with the two sugars due to long CFU growth lags and low maximum specific CFU growth rates of W. sebi at 20°C and due to the fact that growth did not occur at 10°C. At an aw of 0.82, regardless of the temperature, the carrying capacity (i.e., the cell concentration at t = {infty}) of extrawhite sugar was lower than that of white sugar. Together with the fact that the syrup film of extrawhite sugar contained less amino-nitrogen relative to other macronutrients than the syrup film of white sugar, these results suggest that CFU growth of W. sebi in extrawhite sugar may be nitrogen limited. We developed a secondary growth model which is able to predict colony growth lags of W. sebi on syrup agar as a function of temperature and aw. The ability of this model to predict CFU growth lags of W. sebi in crystalline sugar was assessed.

* Corresponding author. Mailing address: Department of Dairy and Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. Phone: 45-3528-3266. Fax: 45-3528-3214. E-mail: na{at}kvl.dk.

Applied and Environmental Microbiology, April 2002, p. 1652-1657, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1652-1657.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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