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Applied and Environmental Microbiology, April 2002, p. 1919-1924, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1919-1924.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Towards Growth of Arbuscular Mycorrhizal Fungi Independent of a Plant Host

Ulrich Hildebrandt, Katharina Janetta, and Hermann Bothe*

Botanisches Institut, Universität zu Köln, D-50923 Cologne, Germany

Received 12 November 2001/ Accepted 21 January 2002

When surface-sterilized spores of the arbuscular mycorrhizal fungus (AMF) Glomus intraradices Sy167 were germinated on agar plates in the slightly modified minimum mineral medium described by G. Bécard and J. A. Fortin (New Phytol. 108:211-218, 1988), slime-forming bacteria, identified as Paenibacillus validus, frequently grew up. These bacteria were able to support growth of the fungus on the agar plates. In the presence of P. validus, hyphae branched profusely and formed coiled structures. These were much more densely packed than the so-called arbuscule-like structures which are formed by AMF grown in coculture with carrot roots transformed with T-DNA from Agrobacterium rhizogenes. The presence of P. validus alone also enabled G. intraradices to form new spores, mainly at the densely packed hyphal coils. The new spores were not as abundant as and were smaller than those formed by AMF in the monoxenic culture with carrot root tissues, but they also contained lipid droplets and a large number of nuclei. In these experiments P. validus could not be replaced by bacteria such as Escherichia coli K-12 or Azospirillum brasilense Sp7. Although no conditions under which the daughter spores regerminate and colonize plants have been found yet, and no factor(s) from P. validus which stimulates fungal growth has been identified, the present findings might be a significant step forward toward growth of AMF independent of any plant host.

* Corresponding author. Mailing address: Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923 Cologne, Germany. Phone: 49 221 470 2760. Fax: 49 221 470 5181. E-mail: Hermann.Bothe{at}uni-koeln.de.

Applied and Environmental Microbiology, April 2002, p. 1919-1924, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1919-1924.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bertaux, J., Schmid, M., Prevost-Boure, N. C., Churin, J. L., Hartmann, A., Garbaye, J., Frey-Klett, P. (2003). In Situ Identification of Intracellular Bacteria Related to Paenibacillus spp. in the Mycelium of the Ectomycorrhizal Fungus Laccaria bicolor S238N. Appl. Environ. Microbiol. 69: 4243-4248 [Abstract] [Full Text]  


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