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Applied and Environmental Microbiology, April 2002, p. 2061-2065, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2061-2065.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment

Michel Pélandakis* and Pierre Pernin

Laboratoire de Biologie Cellulaire, EA 1655, Faculté de Pharmacie, 69373 Lyon Cedex 08, France

Received 10 August 2001/ Accepted 25 January 2002

A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analysis of the PCR product distinguished the main thermophilic Naegleria species from the sampling sites.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire, EA 1655, Faculté de Pharmacie, 8 avenue Rockefeller, 69373, Lyon cedex 08, France. Phone: 33 04 78 77 71 54. Fax: 33 04 78 77 71 58. E-mail: pelandak{at}rockefeller.univ-lyon1.fr.

Applied and Environmental Microbiology, April 2002, p. 2061-2065, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2061-2065.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Robinson, B. S., Monis, P. T., Dobson, P. J. (2006). Rapid, Sensitive, and Discriminating Identification of Naegleria spp. by Real-Time PCR and Melting-Curve Analysis. Appl. Environ. Microbiol. 72: 5857-5863 [Abstract] [Full Text]  


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