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Applied and Environmental Microbiology, April 2002, p. 2071-2076, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2071-2076.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR-Restriction Fragment Length Polymorphism Analysis of a Diagnostic 452-Base-Pair DNA Fragment Discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum Isolates of Human and Animal Origin

K. Guyot,1* A. Follet-Dumoulin,1,2 C. Recourt,1 E. Lelièvre,1 J. C. Cailliez,1,2 and E. Dei-Cas1,3

Ecologie du Parasitisme, IFR 17, Institut Pasteur de Lille, 59019 Lille,1 Laboratoire Environnement et Santé, Université Catholique de Lille, 59046 Lille,2 Parasitologie-Mycologie, Faculté de Médecine et Centre Hospitalier Régional et Universitaire de Lille, 59045 Lille, France3

Received 30 July 2001/ Accepted 15 January 2002

Genomic DNAs from human Cryptosporidium isolates previously typed by analysis of the 18S ribosomal DNA locus (Cryptosporidium parvum bovine genotype, C. parvum human genotype, Cryptosporidium meleagridis, and Cryptosporidium felis) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous Cryptosporidium wrairi sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate Cryptosporidium species and C. parvum genotypes.

* Corresponding author. Mailing address: Ecologie du Parasitisme, Institut Pasteur de Lille, 1 rue du Pr. Calmette, BP 245, 59019 Lille, France. Phone: 33 3 20 87 71 56. Fax: 33 3 20 87 79 08. E-mail: karine.guyot{at}pasteur-lille.fr.

Applied and Environmental Microbiology, April 2002, p. 2071-2076, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2071-2076.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Meamar, A. R., Guyot, K., Certad, G., Dei-Cas, E., Mohraz, M., Mohebali, M., Mohammad, K., Mehbod, A. A., Rezaie, S., Rezaian, M. (2007). Molecular Characterization of Cryptosporidium Isolates from Humans and Animals in Iran. Appl. Environ. Microbiol. 73: 1033-1035 [Abstract] [Full Text]  
  • Xiao, L., Fayer, R., Ryan, U., Upton, S. J. (2004). Cryptosporidium Taxonomy: Recent Advances and Implications for Public Health. Clin. Microbiol. Rev. 17: 72-97 [Abstract] [Full Text]  


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